OBJECTIVE: Oxidative stress contributes significantly to the development of secondary brain injury after intracerebral hemorrhage (ICH). It has been previously demonstrated that hydrogen gas can decrease oxidative stress by scavenging reactive oxygen species. We hypothesized that hydrogen therapy will reduce brain oxidative stress in mice after ICH and thereby will lead to reduced brain edema and improved neurological outcomes. MATERIALS AND METHODS: CD1 male mice (weight 30-35 g) were divided into the following groups: sham, ICH+vehicle (room air), ICH+1-h hydrogen treatment, and ICH+2-h hydrogen treatment. ICH was induced by injection of bacterial collagenase into the right basal ganglia. The evaluation of outcomes was done at two time points: 24 and 72 h post-ICH. Brain water content was measured for assessment of brain edema (wet/dry weight method), and three neurological tests were performed pre- and postoperatively. RESULTS: Collagenase injection was found to induce brain edema and impair functional performance of rats. The hydrogen inhalation reduced these effects acutely (24 h); however it exhibited only a tendency to improvement in the delayed study (72 h). CONCLUSIONS: Our results suggest that hydrogen inhalation exerts an acute brain-protective effect in the mouse ICH model. However, the acute hydrogen therapy alone is not sufficient to improve delayed ICH outcomes in this model.
OBJECTIVE: Oxidative stress contributes significantly to the development of secondary brain injury after intracerebral hemorrhage (ICH). It has been previously demonstrated that hydrogen gas can decrease oxidative stress by scavenging reactive oxygen species. We hypothesized that hydrogen therapy will reduce brain oxidative stress in mice after ICH and thereby will lead to reduced brain edema and improved neurological outcomes. MATERIALS AND METHODS:CD1 male mice (weight 30-35 g) were divided into the following groups: sham, ICH+vehicle (room air), ICH+1-h hydrogen treatment, and ICH+2-h hydrogen treatment. ICH was induced by injection of bacterial collagenase into the right basal ganglia. The evaluation of outcomes was done at two time points: 24 and 72 h post-ICH. Brain water content was measured for assessment of brain edema (wet/dry weight method), and three neurological tests were performed pre- and postoperatively. RESULTS: Collagenase injection was found to induce brain edema and impair functional performance of rats. The hydrogen inhalation reduced these effects acutely (24 h); however it exhibited only a tendency to improvement in the delayed study (72 h). CONCLUSIONS: Our results suggest that hydrogen inhalation exerts an acute brain-protective effect in the mouseICH model. However, the acute hydrogen therapy alone is not sufficient to improve delayed ICH outcomes in this model.
Authors: Marian Valko; Dieter Leibfritz; Jan Moncol; Mark T D Cronin; Milan Mazur; Joshua Telser Journal: Int J Biochem Cell Biol Date: 2006-08-04 Impact factor: 5.085
Authors: Tim Lekic; Richard Hartman; Hugo Rojas; Anatol Manaenko; Wanqiu Chen; Robert Ayer; Jiping Tang; John H Zhang Journal: J Neurotrauma Date: 2010-03 Impact factor: 5.269
Authors: Tim Lekic; Damon Klebe; Roy Poblete; Paul R Krafft; William B Rolland; Jiping Tang; John H Zhang Journal: Curr Med Chem Date: 2015 Impact factor: 4.530
Authors: Katsuhiro Nagata; Kenichiro Kumasaka; Kevin D Browne; Shengjie Li; Jesse St-Pierre; John Cognetti; Joshua Marks; Victoria E Johnson; Douglas H Smith; Jose L Pascual Journal: J Trauma Acute Care Surg Date: 2016-12 Impact factor: 3.313
Authors: Justyna Sosna; Stephan Philipp; Johaiber Fuchslocher Chico; Carina Saggau; Jürgen Fritsch; Alexandra Föll; Johannes Plenge; Christoph Arenz; Thomas Pinkert; Holger Kalthoff; Anna Trauzold; Ingo Schmitz; Stefan Schütze; Dieter Adam Journal: Mol Cell Biol Date: 2016-09-26 Impact factor: 4.272
Authors: Katsuhiro Nagata; Kevin D Browne; Yujin Suto; Kenichiro Kumasaka; John Cognetti; Victoria E Johnson; Joshua Marks; Douglas H Smith; Jose L Pascual Journal: J Trauma Acute Care Surg Date: 2017-09 Impact factor: 3.697
Authors: Katsuhiro Nagata; Yujin Suto; John Cognetti; Kevin D Browne; Kenichiro Kumasaka; Victoria E Johnson; Lewis Kaplan; Joshua Marks; Douglas H Smith; Jose L Pascual Journal: J Trauma Acute Care Surg Date: 2018-05 Impact factor: 3.697