Ali Reza Khalatbary1, Hassan Ahmadvand2. 1. Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khoramabad, Iran. khalat90@yahoo.com 2. Dept. of Biochemistry, Lorestan University zzm321990of Medical Sciences, Khoramabad, Iran.
Abstract
BACKGROUND: Spinal cord injury (SCI) stimulates an inflammatory reaction that causes substantial secondary damage inside the injured spinal tissue. The purpose of this study was to determine the anti-inflammatory effects of epigallocatechin gallate (EGCG) on traumatized spinal cord. METHODS: Rats were randomly divided into four groups of 12 rats each as follow: sham-operated group, trauma group, and EGCG-treatment groups (50 mg/kg, i.p., immediately and 1 hour after SCI). Spinal cord samples were taken 24 hours after injury and studied for determination of myeloperoxidase (MPO) activity, histopathological assessment and immunohistochemistry of tumor necrosis factor-Alpha (TNF-Alpha), interleukin-1Beta (IL-1Beta), Nitrotyrosine, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and poly(ADP-ribose) polymerase (PARP). RESULTS: The results showed that MPO activity was significantly decreased in EGCG-treatment groups. Attenuated TNF-Alphaα, IL-1Beta, Nitrotyrosine, iNOS, COX-2, and PARP expression could be detected in the EGCG treated rats. Also, EGCG attenuated myelin degradation. CONCLUSION: On the basis of these findings, we propose that EGCG may be effective in protecting rat spinal cord from secondary damage by modulating the inflammatory reactions.
BACKGROUND:Spinal cord injury (SCI) stimulates an inflammatory reaction that causes substantial secondary damage inside the injured spinal tissue. The purpose of this study was to determine the anti-inflammatory effects of epigallocatechin gallate (EGCG) on traumatized spinal cord. METHODS:Rats were randomly divided into four groups of 12 rats each as follow: sham-operated group, trauma group, and EGCG-treatment groups (50 mg/kg, i.p., immediately and 1 hour after SCI). Spinal cord samples were taken 24 hours after injury and studied for determination of myeloperoxidase (MPO) activity, histopathological assessment and immunohistochemistry of tumor necrosis factor-Alpha (TNF-Alpha), interleukin-1Beta (IL-1Beta), Nitrotyrosine, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and poly(ADP-ribose) polymerase (PARP). RESULTS: The results showed that MPO activity was significantly decreased in EGCG-treatment groups. Attenuated TNF-Alphaα, IL-1Beta, Nitrotyrosine, iNOS, COX-2, and PARP expression could be detected in the EGCG treated rats. Also, EGCG attenuated myelin degradation. CONCLUSION: On the basis of these findings, we propose that EGCG may be effective in protecting rat spinal cord from secondary damage by modulating the inflammatory reactions.
Authors: T Tonai; Y Taketani; N Ueda; T Nishisho; Y Ohmoto; Y Sakata; M Muraguchi; K Wada; S Yamamoto Journal: J Neurochem Date: 1999-01 Impact factor: 5.372
Authors: Heitor G Araújo-Filho; Lucindo J Quintans-Júnior; André S Barreto; Jackson R G S Almeida; Rosana S S Barreto; Jullyana S S Quintans Journal: Neurochem Res Date: 2015-12-08 Impact factor: 3.996