PURPOSE: This study examined the possible association of G241R and K469E single nucleotide polymorphisms (SNPs) of ICAM-1 gene with the occurrence and clinical/biochemical characteristics of polycystic ovary syndrome (PCOS). METHODS: G241R and K469E SNPs in DNA from peripheral blood leukocytes of 169 PCOS and 259 healthy control women were investigated by real-time PCR combined with melting curve analysis using fluorescence-labeled hybridization probes. RESULTS: We did not find significant differences in the distributions of G241R and K469E polymorphisms, nor in the haplotype frequencies between PCOS and control women. None of the studied polymorphisms were found to affect insulin resistance indices significantly. CONCLUSIONS: These preliminary results suggest that the 241 and 469 SNPs of ICAM-1 gene may not be risk factors for PCOS. Further studies with a larger cohort, analyzing other ICAM-1 polymorphisms are necessary to support our observations before any statement can be made about the relationship between PCOS and ICAM-1 polymorphisms.
PURPOSE: This study examined the possible association of G241R and K469E single nucleotide polymorphisms (SNPs) of ICAM-1 gene with the occurrence and clinical/biochemical characteristics of polycystic ovary syndrome (PCOS). METHODS:G241R and K469E SNPs in DNA from peripheral blood leukocytes of 169 PCOS and 259 healthy control women were investigated by real-time PCR combined with melting curve analysis using fluorescence-labeled hybridization probes. RESULTS: We did not find significant differences in the distributions of G241R and K469E polymorphisms, nor in the haplotype frequencies between PCOS and control women. None of the studied polymorphisms were found to affect insulin resistance indices significantly. CONCLUSIONS: These preliminary results suggest that the 241 and 469 SNPs of ICAM-1 gene may not be risk factors for PCOS. Further studies with a larger cohort, analyzing other ICAM-1 polymorphisms are necessary to support our observations before any statement can be made about the relationship between PCOS and ICAM-1 polymorphisms.
Authors: M Asunción; R M Calvo; J L San Millán; J Sancho; S Avila; H F Escobar-Morreale Journal: J Clin Endocrinol Metab Date: 2000-07 Impact factor: 5.958
Authors: A E Hak; H A Pols; C D Stehouwer; J Meijer; A J Kiliaan; A Hofman; M M Breteler; J C Witteman Journal: J Clin Endocrinol Metab Date: 2001-09 Impact factor: 5.958
Authors: Alberto Francisco Rubio-Guerra; Hilda Vargas-Robles; Alberto Maceda Serrano; Jose Juan Lozano-Nuevo; Bruno Alfonso Escalante-Acosta Journal: Cell Adh Migr Date: 2009-10-07 Impact factor: 3.405
Authors: Syed Douhath Yousuf; Mohammad Ashraf Ganie; Mohammad Afzal Zargar; Shajr Ul Amin; Gulzar A Bhat; Akbar Masood; Fouzia Rashid Journal: Asian Pac J Cancer Prev Date: 2017-11-26