Literature DB >> 2171664

Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae.

B L Kelly1, M L Greenberg.   

Abstract

Phosphatidylglycerophosphatase (EC 3.1.3.27) activity was characterized in mitochondrial extracts from Saccharomyces cerevisiae. The enzyme has a pH optimum of 5.5. Maximum activity occurs in the presence of Triton X-100 (5 mM) and cobalt or magnesium ions (5 mM). The apparent Km for PGP is 14.6 microM. The temperature optimum is between 50 degrees C and 60 degrees C. The enzyme is labile above 50 degrees C. The presence of inositol in growth media results in a slight but reproducible increase in PGPase activity in mitochondrial extracts from glucose-grown cells but not glycerol-grown cells. The inositol effect is not seen in crude cell extracts. Carbon source does not affect PGPase activity in mitochondrial extracts or in crude cell extracts.

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Year:  1990        PMID： 2171664     DOI： 10.1016/0005-2760(90)90181-v

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  21 in total

1.  Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes.

Authors:  M Elkhaimi; M R Kaadige; D Kamath; J C Jackson; H Biliran; J M Lopes
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

2.  Derepression of INO1 transcription requires cooperation between the Ino2p-Ino4p heterodimer and Cbf1p and recruitment of the ISW2 chromatin-remodeling complex.

Authors:  Ameet Shetty; John M Lopes
Journal:  Eukaryot Cell       Date:  2010-10-08

3.  Analysis of Opi1p repressor mutants.

Authors:  Mohan R Kaadige; John M Lopes
Journal:  Curr Genet       Date:  2005-12-02       Impact factor: 3.886

Review 4.  Genetic regulation of phospholipid biosynthesis in Saccharomyces cerevisiae.

Authors:  M L Greenberg; J M Lopes
Journal:  Microbiol Rev       Date:  1996-03

5.  A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4.

Authors:  Christof Osman; Mathias Haag; Felix T Wieland; Britta Brügger; Thomas Langer
Journal:  EMBO J       Date:  2010-05-18       Impact factor: 11.598

6.  Genomic analysis of the Opi- phenotype.

Authors:  Leandria C Hancock; Ryan P Behta; John M Lopes
Journal:  Genetics       Date:  2006-04-02       Impact factor: 4.562

7.  Stimulation of phosphatidylglycerolphosphate phosphatase activity by unsaturated fatty acids in rat heart.

Authors:  S G Cao; G M Hatch
Journal:  Lipids       Date:  1994-07       Impact factor: 1.880

8.  Genomic analysis of PIS1 gene expression.

Authors:  Mary E Gardocki; Margaret Bakewell; Deepa Kamath; Kelly Robinson; Kathy Borovicka; John M Lopes
Journal:  Eukaryot Cell       Date:  2005-03

9.  Regulation of phosphatidylglycerolphosphate synthase in Saccharomyces cerevisiae by factors affecting mitochondrial development.

Authors:  P M Gaynor; S Hubbell; A J Schmidt; R A Lina; S A Minskoff; M L Greenberg
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

10.  Regulation of cardiolipin synthase levels in Saccharomyces cerevisiae.

Authors:  Xuefeng Su; William Dowhan
Journal:  Yeast       Date:  2006-03       Impact factor: 3.239
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