Literature DB >> 21715593

Performance of the LightCycler SeptiFast test Mgrade in detecting microbial pathogens in purulent fluids.

Silvia Sancho-Tello1, Dayana Bravo, Rafael Borrás, Elisa Costa, Beatriz Muñoz-Cobo, David Navarro.   

Abstract

The performance of the LightCycler SeptiFast (SF) assay was compared to that of culture methods in the detection of microorganisms in 43 purulent fluids from patients with pyogenic infections. The SF assay was more sensitive than the culture methods (86% versus 61%, respectively), irrespective of whether the infections were mono- or polymicrobial.

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Year:  2011        PMID： 21715593      PMCID： PMC3147784          DOI： 10.1128/JCM.00359-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Evaluation of the LightCycler SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis.

Authors:  J P Casalta; F Gouriet; V Roux; F Thuny; G Habib; D Raoult
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-12-02       Impact factor: 3.267

2.  Diagnosis of bloodstream infections in immunocompromised patients by real-time PCR.

Authors:  Stefania Varani; Marta Stanzani; Michela Paolucci; Fraia Melchionda; Gastone Castellani; Lorenzo Nardi; Maria Paola Landini; Michele Baccarani; Andrea Pession; Vittorio Sambri
Journal:  J Infect       Date:  2009-03-13       Impact factor: 6.072

3.  Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients.

Authors:  Dayana Bravo; José Blanquer; Mar Tormo; Gerardo Aguilar; Rafael Borrás; Carlos Solano; María A Clari; Elisa Costa; Beatriz Muñoz-Cobo; Mónica Argüeso; José Roberto Pineda; David Navarro
Journal:  Int J Infect Dis       Date:  2011-02-24       Impact factor: 3.623

4.  Identification of bacterial DNA in neutrocytic and non-neutrocytic cirrhotic ascites by means of a multiplex polymerase chain reaction.

Authors:  Tony Bruns; Svea Sachse; Eberhard Straube; Sentayehu Assefa; Andreas Herrmann; Stefan Hagel; Marc Lehmann; Andreas Stallmach
Journal:  Liver Int       Date:  2009-07-07       Impact factor: 5.828

5.  Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia.

Authors:  Marie von Lilienfeld-Toal; Lutz E Lehmann; Ansgar D Raadts; Corinna Hahn-Ast; Katjana S Orlopp; Günter Marklein; Ingvill Purr; Gordon Cook; Andreas Hoeft; Axel Glasmacher; Frank Stüber
Journal:  J Clin Microbiol       Date:  2009-07-01       Impact factor: 5.948

6.  Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis.

Authors:  H Westh; G Lisby; F Breysse; B Böddinghaus; M Chomarat; V Gant; A Goglio; A Raglio; H Schuster; F Stuber; H Wissing; A Hoeft
Journal:  Clin Microbiol Infect       Date:  2009-04-21       Impact factor: 8.067

7.  Multiplex PCR to diagnose bloodstream infections in patients admitted from the emergency department with sepsis.

Authors:  Ephraim L Tsalik; Daphne Jones; Bradly Nicholson; Lynette Waring; Oliver Liesenfeld; Lawrence P Park; Seth W Glickman; Lauren B Caram; Raymond J Langley; Jennifer C van Velkinburgh; Charles B Cairns; Emanuel P Rivers; Ronny M Otero; Stephen F Kingsmore; Tahaniyat Lalani; Vance G Fowler; Christopher W Woods
Journal:  J Clin Microbiol       Date:  2009-10-21       Impact factor: 5.948

8.  Potential clinical utility of polymerase chain reaction in microbiological testing for sepsis.

Authors:  Lutz Eric Lehmann; Julian Alvarez; Klaus-Peter Hunfeld; Antonio Goglio; Gerald J Kost; Richard F Louie; Annibale Raglio; Benito J Regueiro; Heimo Wissing; Frank Stüber
Journal:  Crit Care Med       Date:  2009-12       Impact factor: 7.598

9.  Rapid PCR-based diagnosis of septic arthritis by early Gram-type classification and pathogen identification.

Authors:  Samuel Yang; Padmini Ramachandran; Andrew Hardick; Yu-Hsiang Hsieh; Celeste Quianzon; Marcos Kuroki; Justin Hardick; Aleksandar Kecojevic; Avanthi Abeygunawardena; Jonathan Zenilman; Johan Melendez; Vishal Doshi; Charlotte Gaydos; Richard E Rothman
Journal:  J Clin Microbiol       Date:  2008-02-27       Impact factor: 5.948

10.  Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis.

Authors:  Christine Dierkes; Boris Ehrenstein; Sylvia Siebig; Hans-Jörg Linde; Udo Reischl; Bernd Salzberger
Journal:  BMC Infect Dis       Date:  2009-08-11       Impact factor: 3.090
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  9 in total

1.  Improvement of detection of bacterial pathogens in normally sterile body sites with a focus on orthopedic samples by use of a commercial 16S rRNA broad-range PCR and sequence analysis.

Authors:  K Grif; I Heller; W M Prodinger; K Lechleitner; C Lass-Flörl; D Orth
Journal:  J Clin Microbiol       Date:  2012-05-02       Impact factor: 5.948

2.  Molecular diagnosis of sepsis: New aspects and recent developments.

Authors:  O Liesenfeld; L Lehman; K-P Hunfeld; G Kost
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2014-03-14

Review 3.  Commercial multiplex technologies for the microbiological diagnosis of sepsis.

Authors:  Evan E Lebovitz; Peter D Burbelo
Journal:  Mol Diagn Ther       Date:  2013-08       Impact factor: 4.074

4.  Imaging Single Bacterial Cells with Electro-optical Impedance Microscopy.

Authors:  Fenni Zhang; Shaopeng Wang; Yunze Yang; Jiapei Jiang; Nongjian Tao
Journal:  ACS Sens       Date:  2020-06-04       Impact factor: 7.711

Review 5.  Bench-to-bedside review: Rapid molecular diagnostics for bloodstream infection--a new frontier?

Authors:  Arash Afshari; Jacques Schrenzel; Margareta Ieven; Stephan Harbarth
Journal:  Crit Care       Date:  2012-05-29       Impact factor: 9.097

6.  Multiplex PCR performed of bronchoalveolar lavage fluid increases pathogen identification rate in critically ill patients with pneumonia: a pilot study.

Authors:  Jean-Luc Baudel; Jacques Tankovic; Redouane Dahoumane; Fabrice Carrat; Arnaud Galbois; Hafid Ait-Oufella; Georges Offenstadt; Bertrand Guidet; Eric Maury
Journal:  Ann Intensive Care       Date:  2014-11-25       Impact factor: 6.925

Review 7.  Fungal infections diagnosis - Past, present and future.

Authors:  Alexandre Mendonça; Helena Santos; Ricardo Franco-Duarte; Paula Sampaio
Journal:  Res Microbiol       Date:  2021-12-01       Impact factor: 3.946

Review 8.  Clinical relevance of molecular testing methods in the diagnosis and guidance of therapy in patients with staphylococcal empyema: a systematic review and meta-analysis.

Authors:  Suvash Chandra Ojha; Ke Chen; Yue Yuan; Sarfraz Ahmed; Aijaz Ahmad Malik; Mehru Nisha; Yun-Jian Sheng; Changfeng Sun; Gang Wu; Cun-Liang Deng
Journal:  Front Cell Infect Microbiol       Date:  2022-07-29       Impact factor: 6.073

Review 9.  Identification and Antibiotic-Susceptibility Profiling of Infectious Bacterial Agents: A Review of Current and Future Trends.

Authors:  Gaetano Maugeri; Iana Lychko; Rita Sobral; Ana C A Roque
Journal:  Biotechnol J       Date:  2018-08-26       Impact factor: 4.677
  9 in total

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