Caterina Longo1, Cintia Rito2, Francesca Beretti3, Anna Maria Cesinaro4, Juan Piñeiro-Maceira2, Stefania Seidenari1, Giovanni Pellacani5. 1. Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy. 2. Department of Pathology, University Federal of Rio de Janeiro, Rio de Janeiro, Brazil. 3. Department of Anatomy and Histology, University of Modena and Reggio Emilia, Modena, Italy. 4. Department of Pathology, University of Modena and Reggio Emilia, Modena, Italy. 5. Department of Dermatology, University of Modena and Reggio Emilia, Modena, Italy. Electronic address: pellacani.giovanni@unimore.it.
Abstract
BACKGROUND: Although in the majority of melanomas there is no evidence of pre-existing melanocytic nevus, it is believed that malignant transformation may sometimes occur within a benign precursor. OBJECTIVES: We sought to describe the morphologic features of de novo melanoma and melanoma arising from nevi by means of in vivo confocal microscopy, and to correlate them with their corresponding histopathologic features. METHODS: A total of 113 consecutive, histopathologically proven melanoma cases, 33 arising from a nevus and 80 occurring de novo, were imaged by confocal microscopy and retrospectively evaluated. Cyto-architectural features preferentially expressed in melanomas arising from nevi and in de novo melanomas were defined. RESULTS: By confocal microscopy, abrupt transition, localized distribution of junctional atypical cells, and the presence of dense dermal nests were the most helpful criteria for categorizing a melanoma as arising from a nevus. Melanomas arising from common and congenital nevi were predominantly composed of roundish, monomorphous cells, whereas melanomas arising either de novo or from dysplastic nevi were characterized by markedly pleomorphic cells. LIMITATIONS: The study is retrospective. CONCLUSION: Confocal microscopy is effective in identifying melanoma even when a nevus is simultaneously present, confirming the clinical usefulness of this methodology. Moreover, distinctive features were observed in de novo melanomas and melanomas arising from nevi, permitting accurate distinction between the two groups. Finally, differences in cell morphology, easily detectable by confocal microscopy, seemed to characterize different melanoma types.
BACKGROUND: Although in the majority of melanomas there is no evidence of pre-existing melanocytic nevus, it is believed that malignant transformation may sometimes occur within a benign precursor. OBJECTIVES: We sought to describe the morphologic features of de novo melanoma and melanoma arising from nevi by means of in vivo confocal microscopy, and to correlate them with their corresponding histopathologic features. METHODS: A total of 113 consecutive, histopathologically proven melanoma cases, 33 arising from a nevus and 80 occurring de novo, were imaged by confocal microscopy and retrospectively evaluated. Cyto-architectural features preferentially expressed in melanomas arising from nevi and in de novo melanomas were defined. RESULTS: By confocal microscopy, abrupt transition, localized distribution of junctional atypical cells, and the presence of dense dermal nests were the most helpful criteria for categorizing a melanoma as arising from a nevus. Melanomas arising from common and congenital nevi were predominantly composed of roundish, monomorphous cells, whereas melanomas arising either de novo or from dysplastic nevi were characterized by markedly pleomorphic cells. LIMITATIONS: The study is retrospective. CONCLUSION: Confocal microscopy is effective in identifying melanoma even when a nevus is simultaneously present, confirming the clinical usefulness of this methodology. Moreover, distinctive features were observed in de novo melanomas and melanomas arising from nevi, permitting accurate distinction between the two groups. Finally, differences in cell morphology, easily detectable by confocal microscopy, seemed to characterize different melanoma types.
Authors: C Leikam; A L Hufnagel; C Otto; D J Murphy; B Mühling; S Kneitz; I Nanda; M Schmid; T U Wagner; S Haferkamp; E-B Bröcker; M Schartl; S Meierjohann Journal: Cell Death Dis Date: 2015-04-02 Impact factor: 8.469