| Literature DB >> 21713639 |
Sally L Gras1, Lynne J Waddington, Kenneth N Goldie.
Abstract
Transmission Electron Microscopy of negatively stained and cryo-prepared specimens allows amyloid fibrils to be visualised at high resolution in a dried or a hydrated state, and is an essential method for characterising the morphology of fibrils and pre-fibrillar species. We outline the key steps involved in the preparation and observation of samples using negative staining and cryo-electron preservation. We also discuss methods to measure fibril characteristics, such as fibril width, from electron micrographs.Mesh：
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Year: 2011 PMID： 21713639 DOI： 10.1007/978-1-60327-223-0_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745