Herpes simplex virus type 1 mutants for the origin-binding protein induce DNA amplification in the absence of viral replication.

Herpes simplex virus (HSV) induces DNA amplification within the host cell genome, which is mediated by a set of six of seven HSV replication genes. The origin-binding protein (UL9) is dispensible. By the use of HSV mutants for the UL9 gene we show here that HSV can induce DNA amplification in the absence of lytic viral growth in contrast to replication-negative mutants for either the UL8 or UL52 gene used as control. The amplification-inducing potential of HSV may be relevant for the pathogenicity of the virus.