OBJECTIVE: To sift and identify the nephrotoxic components in Zexie for controlling the quality of the herb. METHOD: The fractions of zexie were prepared by Pre-HPLC, then the nephrotoxicity of the fraction was sifted using LLC-PK1 labelled with fluorescein diacetate and MTT assay. Finally, the compounds in the most obvious nephrotoxic fraction were identified with LC-MS. RESULT: Using MTT and FDA assay, similar results were obtained. Fraction C13 was found to be the most toxic with FDA assay, in which three compounds, alisol C, 16, 23-oxido-alisol B and alisol O, were detected and characterized by multi -stage mass spectrometric analysis. CONCLUSION: Alisol C, 16, 23-oxido-alisol B and alisol O in Zexie may cause nephrotoxicity.
OBJECTIVE: To sift and identify the nephrotoxic components in Zexie for controlling the quality of the herb. METHOD: The fractions of zexie were prepared by Pre-HPLC, then the nephrotoxicity of the fraction was sifted using LLC-PK1 labelled with fluorescein diacetate and MTT assay. Finally, the compounds in the most obvious nephrotoxic fraction were identified with LC-MS. RESULT: Using MTT and FDA assay, similar results were obtained. Fraction C13 was found to be the most toxic with FDA assay, in which three compounds, alisol C, 16, 23-oxido-alisol B and alisol O, were detected and characterized by multi -stage mass spectrometric analysis. CONCLUSION:Alisol C, 16, 23-oxido-alisol B and alisol O in Zexie may cause nephrotoxicity.