OBJECTIVE: To develop an UPLC method of determining the characteristic chromatographic profile of Persicae Semen for controlling the drug quality quickly and accurately. METHOD: The UPLC characteristic chromatographic profiles of fifteen batches of Persicae Semen were determined on an HSS T3 column (2.1 mm x 100 mm, 1.8 microm) eluted with the mobile phase consisted of acetonitrile-water containing 0.05% phosphoric acid in gradient mode and the detection wavelength was at 254 nm. RESULT: The common mode of the UPLC characteristic chromatographic profile was set up. There were 12 common peaks in the fingerprints of fifteen samples, six of which were identified, and the similar degrees of the fifteen batches to the common mode were between 0.884-0.996. CONCLUSION: The method was quick and accurate. The characteristic chromatographic profile of Persicae Semen with high specificity can be used to control the quality of Persicae Semen.
OBJECTIVE: To develop an UPLC method of determining the characteristic chromatographic profile of Persicae Semen for controlling the drug quality quickly and accurately. METHOD: The UPLC characteristic chromatographic profiles of fifteen batches of Persicae Semen were determined on an HSS T3 column (2.1 mm x 100 mm, 1.8 microm) eluted with the mobile phase consisted of acetonitrile-water containing 0.05% phosphoric acid in gradient mode and the detection wavelength was at 254 nm. RESULT: The common mode of the UPLC characteristic chromatographic profile was set up. There were 12 common peaks in the fingerprints of fifteen samples, six of which were identified, and the similar degrees of the fifteen batches to the common mode were between 0.884-0.996. CONCLUSION: The method was quick and accurate. The characteristic chromatographic profile of Persicae Semen with high specificity can be used to control the quality of Persicae Semen.