| Literature DB >> 21708158 |
Elisabeth Marchal1, Liesbeth Badisco, Heleen Verlinden, Tim Vandersmissen, Sofie Van Soest, Pieter Van Wielendaele, Jozef Vanden Broeck.
Abstract
The functional characterization of the Halloween genes represented a major breakthrough in the elucidation of the ecdysteroid biosynthetic pathway. These genes encode cytochrome P450 enzymes catalyzing the final steps of ecdysteroid biosynthesis in the dipteran Drosophila melanogaster and the Lepidoptera Manduca sexta and Bombyx mori. This is the first report on the identification of two Halloween genes, spook (spo) and phantom (phm), from a hemimetabolous orthopteran insect, the desert locust Schistocerca gregaria. Using q-RT-PCR, their spatial and temporal transcript profiles were analyzed in both final larval stage and adult locusts. The circulating ecdysteroid titers in the hemolymph were measured and found to correlate well with changes in the temporal transcript profiles of spo and phm. Moreover, an RNA interference (RNAi)-based approach was employed to study knockdown effects upon silencing of both transcripts in the fifth larval stage. Circulating ecdysteroid levels were found to be significantly reduced upon dsRNA treatment.Entities:
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Year: 2011 PMID： 21708158 DOI： 10.1016/j.jinsphys.2011.05.009
Source DB: PubMed Journal: J Insect Physiol ISSN： 0022-1910 Impact factor: 2.354