OBJECTIVE: To evaluate the efficacy and diagnostic potential of interferon- gamma release assays QuantiFERON-TB Gold® In Tube (QFT-TB-IT) for the detection of gamma interferon against the antigen ESAT-6, CFP-10, TB 7.7(p4) of Mycobacterium tuberculosis and compare the reactivity with other methods (smear, culture and IS6110 targeting PCR). METHODS: The study was carried out on children with pulmonary and extra pulmonary tuberculosis along with matching and PPD+ controls (82 cases, 48 matching controls and 20 PPD+ controls). RESULTS: QFT-TB-IT showed a significantly higher sensitivity (51.2%) as compared with LJ medium culture method (11.0%; p < 0.001), BacT/Alert 3D system (12.1%; p < 0.001), ZN staining (19.5%; p < 0.001) and PCR (45.1%; p < 0.05). Specificity of QFT-TB-IT was 48.0%. CONCLUSIONS: Less specificity of this assay may be due to high endemicity of tuberculosis. However, further studies are recommended.
OBJECTIVE: To evaluate the efficacy and diagnostic potential of interferon- gamma release assays QuantiFERON-TB Gold® In Tube (QFT-TB-IT) for the detection of gamma interferon against the antigen ESAT-6, CFP-10, TB 7.7(p4) of Mycobacterium tuberculosis and compare the reactivity with other methods (smear, culture and IS6110 targeting PCR). METHODS: The study was carried out on children with pulmonary and extra pulmonary tuberculosis along with matching and PPD+ controls (82 cases, 48 matching controls and 20 PPD+ controls). RESULTS: QFT-TB-IT showed a significantly higher sensitivity (51.2%) as compared with LJ medium culture method (11.0%; p < 0.001), BacT/Alert 3D system (12.1%; p < 0.001), ZN staining (19.5%; p < 0.001) and PCR (45.1%; p < 0.05). Specificity of QFT-TB-IT was 48.0%. CONCLUSIONS: Less specificity of this assay may be due to high endemicity of tuberculosis. However, further studies are recommended.
Authors: Pernille Ravn; Martin E Munk; Ase B Andersen; Bettina Lundgren; Jens D Lundgren; Lars N Nielsen; Axel Kok-Jensen; Peter Andersen; Karin Weldingh Journal: Clin Diagn Lab Immunol Date: 2005-04
Authors: Madhukar Pai; Kaustubh Gokhale; Rajnish Joshi; Sandeep Dogra; Shriprakash Kalantri; Deepak K Mendiratta; Pratibha Narang; Charles L Daley; Reuben M Granich; Gerald H Mazurek; Arthur L Reingold; Lee W Riley; John M Colford Journal: JAMA Date: 2005-06-08 Impact factor: 56.272
Authors: Noëmi R Meier; Thomas Volken; Marc Geiger; Ulrich Heininger; Marc Tebruegge; Nicole Ritz Journal: Front Pediatr Date: 2019-05-29 Impact factor: 3.418