PRIMARY OBJECTIVE: To investigate the relationship between human epidermal growth factor receptor (HER)-2/neu and the gene encoding topoisomerase IIα (TOP2A) in breast cancer, while elucidating their association with clinicopathological variables. METHODS: Real-time quantitative polymerase chain reaction (RQ-PCR) was performed on a 96-patient study group to assess gene amplification, and levels were determined using the comparative cycle threshold approach and Taqman assays. An immunohistochemistry (IHC) microarray (n = 76) was then employed to check for correlation between gene amplification and protein expression levels. RESULTS: Amplification levels of TOP2A did not differ significantly according to HER-2/neu status by either RQ-PCR or IHC microarray. Of the HER-2/neu(-) patients, 29.1% demonstrated levels of TOP2A above the third quartile, whereas 22.9% of the HER-2/neu(+) patients had values in the first quartile (log TOP2A <0.62), thereby indicating low-level amplification. Of the 60 patients characterized as HER-2/neu(-) using IHC and fluorescence in situ hybridization (FISH), 22.9% were classified as TOP2A(+) on the IHC microarray. Of the 14 patients deemed HER-2/neu(+) using IHC and FISH, meanwhile, the majority (n = 10) were classified as TOP2A(+). CONCLUSIONS: Our results indicate that amplification of TOP2A in breast cancer is not confined to those who are concomitantly HER-2/neu(+), and suggest that a significant proportion of HER-2/neu(-) patients exhibit high levels of TOP2A.
PRIMARY OBJECTIVE: To investigate the relationship between humanepidermal growth factor receptor (HER)-2/neu and the gene encoding topoisomerase IIα (TOP2A) in breast cancer, while elucidating their association with clinicopathological variables. METHODS: Real-time quantitative polymerase chain reaction (RQ-PCR) was performed on a 96-patient study group to assess gene amplification, and levels were determined using the comparative cycle threshold approach and Taqman assays. An immunohistochemistry (IHC) microarray (n = 76) was then employed to check for correlation between gene amplification and protein expression levels. RESULTS: Amplification levels of TOP2A did not differ significantly according to HER-2/neu status by either RQ-PCR or IHC microarray. Of the HER-2/neu(-) patients, 29.1% demonstrated levels of TOP2A above the third quartile, whereas 22.9% of the HER-2/neu(+) patients had values in the first quartile (log TOP2A <0.62), thereby indicating low-level amplification. Of the 60 patients characterized as HER-2/neu(-) using IHC and fluorescence in situ hybridization (FISH), 22.9% were classified as TOP2A(+) on the IHC microarray. Of the 14 patients deemed HER-2/neu(+) using IHC and FISH, meanwhile, the majority (n = 10) were classified as TOP2A(+). CONCLUSIONS: Our results indicate that amplification of TOP2A in breast cancer is not confined to those who are concomitantly HER-2/neu(+), and suggest that a significant proportion of HER-2/neu(-) patients exhibit high levels of TOP2A.
Authors: Virginie Durbecq; Marianne Paesmans; Fatima Cardoso; Christine Desmedt; Angelo Di Leo; Stephen Chan; Kay Friedrichs; Tamas Pinter; Simon Van Belle; Elizabeth Murray; István Bodrogi; Euan Walpole; Bernard Lesperance; Stefan Korec; John Crown; Peter Simmonds; Thimothy J Perren; Jean-Yves Leroy; Ghizlane Rouas; Christos Sotiriou; Martine Piccart; Denis Larsimont Journal: Mol Cancer Ther Date: 2004-10 Impact factor: 6.261
Authors: F Arcamone; S Penco; A Vigevani; S Redaelli; G Franchi; A DiMarco; A M Casazza; T Dasdia; F Formelli; A Necco; C Soranzo Journal: J Med Chem Date: 1975-07 Impact factor: 7.446
Authors: H B Muss; A D Thor; D A Berry; T Kute; E T Liu; F Koerner; C T Cirrincione; D R Budman; W C Wood; M Barcos Journal: N Engl J Med Date: 1994-05-05 Impact factor: 91.245
Authors: Anna J Zaczek; Aleksandra Markiewicz; Barbara Seroczynska; Jaroslaw Skokowski; Janusz Jaskiewicz; Tadeusz Pienkowski; Wojciech P Olszewski; Jolanta Szade; Piotr Rhone; Marzena Welnicka-Jaskiewicz; Jacek Jassem Journal: Oncologist Date: 2012-08-07
Authors: Rosa Nadal; Ana Fernandez; Pedro Sanchez-Rovira; Marta Salido; María Rodríguez; José Luis García-Puche; Marta Macià; Josep Maria Corominas; Miguel Delgado-Rodriguez; Lucas Gonzalez; Joan Albanell; Mónica Fernández; Francesc Solé; José Antonio Lorente; María José Serrano Journal: Breast Cancer Res Date: 2012-05-03 Impact factor: 6.466