Yan Ma1, Yong Tao, Qiang Lu, Yan-Rong Jiang. 1. Department of Ophthalmology, People's Hospital, Peking University, Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China.
Abstract
PURPOSE: Because serum amyloid A can regulate angiogenesis, we searched for an association between serum amyloid A and interleukin-6 (IL-6), as proinflammatory factors, and proliferative diabetic retinopathy (PDR). DESIGN: Retrospective, comparative study. METHODS: Seventy-six patients (76 eyes) with PDR and 31 patients (31 eyes) with nondiabetic ocular disease (control group), including idiopathic epiretinal membranes (8 eyes) and idiopathic macular holes (23 eyes), were enrolled. Enzyme-linked immunosorbent assay, dual-color immunofluorescence staining, and semiquantitative reverse-transcription polymerase chain reaction were used to examine the serum amyloid A and IL-6 levels in vitreous and plasma, expression of protein and mRNA of serum amyloid A in the excised membranes, respectively. RESULTS: Vitreous serum amyloid A and IL-6 levels in the study group were significantly higher than those in the control group (both P < .001), whereas the plasma concentrations of serum amyloid A and IL-6 did not vary significantly between the groups (P = .555 and P = .621, respectively). A significant correlation was observed between the vitreous and plasma levels of serum amyloid A in subjects with PDR (r = 0.525; P < .001). In fibrovascular membranes of the study group, colocalization of endothelial marker CD31 with serum amyloid A and colocalization of fibrillar structure markers fibronectin with serum amyloid A were observed. Expression of serum amyloid A mRNA was significantly higher in fibrovascular membranes with PDR than in idiopathic epiretinal membranes (P = .004). CONCLUSIONS: Serum amyloid A and IL-6 may be involved with the inflammatory process in the development of PDR. Local expression of serum amyloid A may exist in PDR.
PURPOSE: Because serum amyloid A can regulate angiogenesis, we searched for an association between serum amyloid A and interleukin-6 (IL-6), as proinflammatory factors, and proliferative diabetic retinopathy (PDR). DESIGN: Retrospective, comparative study. METHODS: Seventy-six patients (76 eyes) with PDR and 31 patients (31 eyes) with nondiabetic ocular disease (control group), including idiopathic epiretinal membranes (8 eyes) and idiopathic macular holes (23 eyes), were enrolled. Enzyme-linked immunosorbent assay, dual-color immunofluorescence staining, and semiquantitative reverse-transcription polymerase chain reaction were used to examine the serum amyloid A and IL-6 levels in vitreous and plasma, expression of protein and mRNA of serum amyloid A in the excised membranes, respectively. RESULTS: Vitreous serum amyloid A and IL-6 levels in the study group were significantly higher than those in the control group (both P < .001), whereas the plasma concentrations of serum amyloid A and IL-6 did not vary significantly between the groups (P = .555 and P = .621, respectively). A significant correlation was observed between the vitreous and plasma levels of serum amyloid A in subjects with PDR (r = 0.525; P < .001). In fibrovascular membranes of the study group, colocalization of endothelial marker CD31 with serum amyloid A and colocalization of fibrillar structure markers fibronectin with serum amyloid A were observed. Expression of serum amyloid A mRNA was significantly higher in fibrovascular membranes with PDR than in idiopathic epiretinal membranes (P = .004). CONCLUSIONS: Serum amyloid A and IL-6 may be involved with the inflammatory process in the development of PDR. Local expression of serum amyloid A may exist in PDR.
Authors: Dean F Loporchio; Emily K Tam; Jane Cho; Jaeyoon Chung; Gyungah R Jun; Weiming Xia; Marissa G Fiorello; Nicole H Siegel; Steven Ness; Thor D Stein; Manju L Subramanian Journal: Cells Date: 2021-04-30 Impact factor: 6.600
Authors: Federica Storti; Jennifer Pulley; Pascal Kuner; Markus Abt; Ulrich F O Luhmann Journal: Transl Vis Sci Technol Date: 2021-10-04 Impact factor: 3.283
Authors: Craig Balmforth; Job Jmh van Bragt; Titia Ruijs; James R Cameron; Robert Kimmitt; Rebecca Moorhouse; Alicja Czopek; May Khei Hu; Peter J Gallacher; James W Dear; Shyamanga Borooah; Iain M MacIntyre; Tom Mc Pearson; Laura Willox; Dinesh Talwar; Muriel Tafflet; Christophe Roubeix; Florian Sennlaub; Siddharthan Chandran; Baljean Dhillon; David J Webb; Neeraj Dhaun Journal: JCI Insight Date: 2016-12-08