Long Cui1, Yanru Lou2, Xinjun Zhang3, Hui Zhou1, Hongxia Deng1, Haojun Song1, Xiuchong Yu1, Bingxiu Xiao4, Weihua Wang3, Junming Guo5. 1. Ningbo University School of Medicine, Ningbo 315211, China. 2. Ningbo University School of Medicine, Ningbo 315211, China; Affiliated Hospital and Ningbo No. 1 Hospital, Ningbo University School of Medicine, Ningbo 315000, China. 3. Ningbo University School of Medicine, Ningbo 315211, China; Affiliated Hospital, Ningbo University School of Medicine, Ningbo 315010, China. 4. Ningbo University School of Medicine, Ningbo 315211, China. Electronic address: xiaobingxiu@nbu.edu.cn. 5. Ningbo University School of Medicine, Ningbo 315211, China. Electronic address: junmingguo@yahoo.com.
Abstract
OBJECTIVE: To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS: Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in the peripheral blood of 93 patients with gastric cancer and 32 healthy volunteers. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic values. RESULTS: The peripheral blood levels of piR-651 and piR-823 in the patients with gastric cancer were significantly lower than those from controls (P<0.001). The piR-651 level in gastric adenocarcinoma was higher than that in gastric signet ring cell carcinoma (P = 0.003). The piR-823 level was positively associated with tumor-node-metastasis stage (P = 0.027) and distant metastasis (P = 0.026). The areas under the ROC curve were 0.841, 0.812 and 0.860 for piR-651, piR-823 and the combination, respectively. CONCLUSIONS: piRNAs may be valuable biomarkers for detecting circulating gastric cancer cells.
OBJECTIVE: To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancerpatients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS: Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in the peripheral blood of 93 patients with gastric cancer and 32 healthy volunteers. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic values. RESULTS: The peripheral blood levels of piR-651 and piR-823 in the patients with gastric cancer were significantly lower than those from controls (P<0.001). The piR-651 level in gastric adenocarcinoma was higher than that in gastric signet ring cell carcinoma (P = 0.003). The piR-823 level was positively associated with tumor-node-metastasis stage (P = 0.027) and distant metastasis (P = 0.026). The areas under the ROC curve were 0.841, 0.812 and 0.860 for piR-651, piR-823 and the combination, respectively. CONCLUSIONS: piRNAs may be valuable biomarkers for detecting circulating gastric cancer cells.