| Literature DB >> 21703312 |
Jeff A Brady1, Jennifer B Faske, Jessica M Castañeda-Gill, Jonathan L King, Forrest L Mitchell.
Abstract
We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost.Entities:
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Year: 2011 PMID： 21703312 DOI： 10.1016/j.mimet.2011.06.007
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363