| Literature DB >> 21699995 |
Débora C O Nunes1, Renata S Rodrigues, Malson N Lucena, Camila T Cologna, Ana Carolina S Oliveira, Amélia Hamaguchi, Maria I Homsi-Brandeburgo, Eliane C Arantes, David N S Teixeira, Carlos Ueira-Vieira, Veridiana M Rodrigues.
Abstract
In the present study, an acidic PLA(2), designated Bl-PLA(2), was isolated from Bothrops leucurus snake venom through two chromatographic steps: ion-exchange on CM-Sepharose and hydrophobic chromatography on Phenyl-Sepharose. Bl-PLA(2) was homogeneous on SDS-PAGE and when submitted to 2D electrophoresis the molecular mass was 15,000Da and pI was 5.4. Its N-terminal sequence revealed a high homology with other Asp49 acidic PLA(2)s from snake venoms. Its specific activity was 159.9U/mg and the indirect hemolytic activity was also higher than that of the crude venom. Bl-PLA(2) induced low myotoxic and edema activities as compared to those of the crude venom. Moreover, the enzyme was able to induce increments in IL-12p40, TNF-α, IL-1β and IL-6 levels and no variation of IL-8 and IL-10 in human PBMC stimulated in vitro, suggesting that Bl-PLA(2) induces proinflammatory cytokine production by human mononuclear cells. Bothrops leucurus venom is still not extensively explored and knowledge of its components will contribute for a better understanding of its action mechanism.Entities:
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Year: 2011 PMID： 21699995 DOI： 10.1016/j.cbpc.2011.06.003
Source DB: PubMed Journal: Comp Biochem Physiol C Toxicol Pharmacol ISSN： 1532-0456 Impact factor: 3.228