| Literature DB >> 2169672 |
C Lecomte1, J J Pin, L De Moerlooze, D Vandenbergh, A F Lambert, P P Pastoret, G Chappuis.
Abstract
A panel of monoclonal antibodies was prepared by immunization of BALB/c mice with Moredun (BD) virus strains. These antibodies were characterized by immunofluorescence and seroneutralization against BD, BVD and hog cholera (HC) virus strains, and radioimmunoprecipitation of BVD-infected cells extracts. The MAbs reacting with the majority of the Pestivirus strains recognize the 80 kDa antigen of the BVD cytophathic strains. The 80 kDa antigen of the BVD/Osloss virus strain has been cloned and expressed in E. coli as a fusion protein with beta-galactosidase. The fusion protein has been purified from inclusion bodies and used successfully as an antigen for ELISA detection of BVDV specific antibodies in bovine sera. A competitive ELISA using MAbs is more specific than a direct assay. These results compare well with the ones obtained with antigen extracted from BVDV-infected cells.Entities:
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Year: 1990 PMID： 2169672 DOI： 10.1016/0378-1135(90)90149-p
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293