Literature DB >> 2169264

Detection of human papilloma virus DNA sequences by polymerase chain reaction.

F H Sarkar1, J D Crissman.   

Abstract

We have developed a polymerase chain reaction-based procedure for reproducible detection of the E6-E7 gene in human papilloma virus DNA sequences using formalin-fixed, paraffin-embedded tissue sections. This procedure is a simple one-step procedure which does not require any elaborate hybridization following polymerase chain reaction amplification. The protocol combines modified tissue treatment and proper primer selection for efficient amplification of target DNA in a highly specific manner allowing identification in ethidium bromide-stained gels. The procedure described here is useful for a variety of tissue preparations, particularly formalin-fixed, paraffin-embedded archival tissues.

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Year:  1990        PMID： 2169264

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  6 in total

Review 1.  Molecular biology made easy. The polymerase chain reaction.

Authors:  A M Clarke; N P Mapstone; P Quirke
Journal:  Histochem J       Date:  1992-12

2.  Transfection of the inner cell mass and lack of a unique DNA sequence affecting the uptake of exogenous DNA by sperm as shown by dideoxy sequencing analogues.

Authors:  M Cabrera; P J Chan; T H Kalugdan; A King
Journal:  J Assist Reprod Genet       Date:  1997-02       Impact factor: 3.412

3.  Human papilloma virus DNA exposure and embryo survival is stage-specific.

Authors:  Andrew A Henneberg; William C Patton; John D Jacobson; Philip J Chan
Journal:  J Assist Reprod Genet       Date:  2006-07-27       Impact factor: 3.412

4.  Evidence for ease of transmission of human papillomavirus DNA from sperm to cells of the uterus and embryo.

Authors:  P J Chan; I M Seraj; T H Kalugdan; A King
Journal:  J Assist Reprod Genet       Date:  1996-07       Impact factor: 3.412

5.  Evaluation of Cytokines in Endocervical Secretion and Vaginal pH from Women with Bacterial Vaginosis or Human Papillomavirus.

Authors:  Ana Claudia Camargo Campos; Eddie Fernando Candido Murta; Márcia Antoniazi Michelin; Cleomenes Reis
Journal:  ISRN Obstet Gynecol       Date:  2012-03-22

6.  Human papillomavirus types 16 and 18 and the prognosis of patients with stage I cervical cancer.

Authors:  Rossana de Araújo Catão Zampronha; Ruffo Freitas-Junior; Eddie Fernando Candido Murta; Márcia Antoniazi Michelin; Aline Almeida Barbaresco; Sheila Jorge Adad; Amaurillo Monteiro de Oliveira; Amanda B Rassi; Glória Jabur Bittar Oton
Journal:  Clinics (Sao Paulo)       Date:  2013-06       Impact factor: 2.365
  6 in total

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