Literature DB >> 21688279

Protocol for high-resolution electrophoresis separation of myosin heavy chain isoforms in bovine skeletal muscle.

Brigitte Picard1, Christiane Barboiron, David Chadeyron, Catherine Jurie.   

Abstract

In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2011        PMID： 21688279     DOI： 10.1002/elps.201100118

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  9 in total

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Authors:  Aidan P Moloney; Edward G O'Riordan; Mark McGee; Brigitte Picard; Frank J Monahan; Lara Moran; Raquel Cama-Moncunill
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8.  A simplified immunohistochemical classification of skeletal muscle fibres in mouse.

Authors:  M Kammoun; I Cassar-Malek; B Meunier; B Picard
Journal:  Eur J Histochem       Date:  2014-06-24       Impact factor: 3.188

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Authors:  Malek Kammoun; Brigitte Picard; Thierry Astruc; Mohammed Gagaoua; Denise Aubert; Muriel Bonnet; Véronique Blanquet; Isabelle Cassar-Malek
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  9 in total

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