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Literature DB >> 21681199

The role of trimerization in the osmoregulated betaine transporter BetP.

Camilo Perez¹, Kamil Khafizov, Lucy R Forrest, Reinhard Krämer, Christine Ziegler.

Abstract

The osmoregulated betaine transporter BetP is a stable trimer. Structural studies have shown that individual protomers can adopt distinct transport conformations, implying a functional role for the trimeric state in transport, although the role of trimerization in regulation is not yet understood. We designed putative monomeric mutants by molecular-dynamics simulations and in silico alanine-scanning mutagenesis. Several mutants including BetP-W101A/T351A were monomeric in detergent as well as in the membrane, as shown by blue native gel electrophoresis, crosslinking and electron microscopy. This monomeric form retains the ability to accumulate betaine, but is no longer regulated by hyperosmotic shock.

Entities: Chemical Disease Gene Mutation

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Year: 2011 PMID： 21681199 PMCID： PMC3147255 DOI： 10.1038/embor.2011.102

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

23 in total

1. A simple physical model for binding energy hot spots in protein-protein complexes.

Authors: Tanja Kortemme; David Baker
Journal: Proc Natl Acad Sci U S A Date: 2002-10-15 Impact factor: 11.205

2. Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport.

Authors: Satoshi Murakami; Norihisa Tamura; Asami Saito; Takahiro Hirata; Akihito Yamaguchi
Journal: J Biol Chem Date: 2003-10-23 Impact factor: 5.157

Review 3. Computational design of protein-protein interactions.

Authors: Tanja Kortemme; David Baker
Journal: Curr Opin Chem Biol Date: 2004-02 Impact factor: 8.822

4. Structural asymmetry in a trimeric Na+/betaine symporter, BetP, from Corynebacterium glutamicum.

Authors: Ching-Ju Tsai; Kamil Khafizov; Jonna Hakulinen; Lucy R Forrest; Lucy R Forrest; Reinhard Krämer; Werner Kühlbrandt; Christine Ziegler
Journal: J Mol Biol Date: 2011-01-31 Impact factor: 5.469

5. Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters.

Authors: Atsuko Yamashita; Satinder K Singh; Toshimitsu Kawate; Yan Jin; Eric Gouaux
Journal: Nature Date: 2005-07-24 Impact factor: 49.962

6. Analysis of integral membrane protein contributions to the deformability and stability of the human erythrocyte membrane.

Authors: H M Van Dort; D W Knowles; J A Chasis; G Lee; N Mohandas; P S Low
Journal: J Biol Chem Date: 2001-10-10 Impact factor: 5.157

7. Osmo-sensing by N- and C-terminal extensions of the glycine betaine uptake system BetP of Corynebacterium glutamicum.

Authors: H Peter; A Burkovski; R Krämer
Journal: J Biol Chem Date: 1998-01-30 Impact factor: 5.157

8. Glycine betaine uptake after hyperosmotic shift in Corynebacterium glutamicum.

Authors: M Farwick; R M Siewe; R Krämer
Journal: J Bacteriol Date: 1995-08 Impact factor: 3.490

9. The C-terminal domain of the betaine carrier BetP of Corynebacterium glutamicum is directly involved in sensing K+ as an osmotic stimulus.

Authors: Dirk Schiller; René Rübenhagen; Reinhard Krämer; Susanne Morbach
Journal: Biochemistry Date: 2004-05-18 Impact factor: 3.162

10. Projection structure and oligomeric state of the osmoregulated sodium/glycine betaine symporter BetP of Corynebacterium glutamicum.

Authors: C Ziegler; S Morbach; D Schiller; R Krämer; C Tziatzios; D Schubert; W Kühlbrandt
Journal: J Mol Biol Date: 2004-04-09 Impact factor: 5.469

15 in total

1. Trimerization of dopamine transporter triggered by AIM-100 binding: Molecular mechanism and effect of mutations.

Authors: Mary Hongying Cheng; Luca Ponzoni; Tatiana Sorkina; Ji Young Lee; She Zhang; Alexander Sorkin; Ivet Bahar
Journal: Neuropharmacology Date: 2019-06-20 Impact factor: 5.250

2. Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling.

Authors: Camilo Perez; Belinda Faust; Ahmad Reza Mehdipour; Kevin A Francesconi; Lucy R Forrest; Christine Ziegler
Journal: Nat Commun Date: 2014-07-15 Impact factor: 14.919

3. Ectoine and hydroxyectoine as protectants against osmotic and cold stress: uptake through the SigB-controlled betaine-choline- carnitine transporter-type carrier EctT from Virgibacillus pantothenticus.

Authors: Anne U Kuhlmann; Tamara Hoffmann; Jan Bursy; Mohamed Jebbar; Erhard Bremer
Journal: J Bacteriol Date: 2011-07-15 Impact factor: 3.490

The role of trimerization in the osmoregulated betaine transporter BetP.

1. A simple physical model for binding energy hot spots in protein-protein complexes.

2. Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport.

Review 3. Computational design of protein-protein interactions.

4. Structural asymmetry in a trimeric Na+/betaine symporter, BetP, from Corynebacterium glutamicum.

5. Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters.

6. Analysis of integral membrane protein contributions to the deformability and stability of the human erythrocyte membrane.

7. Osmo-sensing by N- and C-terminal extensions of the glycine betaine uptake system BetP of Corynebacterium glutamicum.

8. Glycine betaine uptake after hyperosmotic shift in Corynebacterium glutamicum.

9. The C-terminal domain of the betaine carrier BetP of Corynebacterium glutamicum is directly involved in sensing K+ as an osmotic stimulus.

10. Projection structure and oligomeric state of the osmoregulated sodium/glycine betaine symporter BetP of Corynebacterium glutamicum.

1. Trimerization of dopamine transporter triggered by AIM-100 binding: Molecular mechanism and effect of mutations.

2. Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling.

3. Ectoine and hydroxyectoine as protectants against osmotic and cold stress: uptake through the SigB-controlled betaine-choline- carnitine transporter-type carrier EctT from Virgibacillus pantothenticus.

4. Properties of an inward-facing state of LeuT: conformational stability and substrate release.

Review 5. The Cardiac Na⁺ -Ca²⁺ Exchanger: From Structure to Function.

6. Lipid-Protein Interactions in the Regulated Betaine Symporter BetP Probed by Infrared Spectroscopy.

7. Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP.

8. Structural evidence for functional lipid interactions in the betaine transporter BetP.

9. Alternating-access mechanism in conformationally asymmetric trimers of the betaine transporter BetP.

Review 10. The Role of the Membrane in Transporter Folding and Activity.

Review 3. Computational design of protein-protein interactions.

Review 5. The Cardiac Na+ -Ca2+ Exchanger: From Structure to Function.

Review 10. The Role of the Membrane in Transporter Folding and Activity.

Review 5. The Cardiac Na⁺ -Ca²⁺ Exchanger: From Structure to Function.