BACKGROUND & AIMS: Probiotic bacteria are used as food supplement in many different disease settings. The immune modulating capacity of different strains is not always properly tested which might result in a suboptimal choice of strains for clinical use. METHODS: The CD4 T cell responses to 19 different gut derived lactic acid bacteria were tested with different methods to show their diversity in immune modulation and to make a well-founded choice on which strains to use in future clinical trials. After co-culture of PBMC with bacteria, the induction of CD4(+) T cell subsets (regulatory T cells, T helper type (TH)1, TH2 and TH17) was analysed by rtPCR of transcription factor mRNA, intracellular FACS staining of transcription factors and cytokine production. RESULTS: Bacterial strains all have diverse, unique immune modulatory properties. Strains can induce Treg, TH1, TH2 and TH17 cells which can be shown at different levels of T cell activation, and is consistent for most strains tested. For TH1, TH17 and Treg, a positive correlation between the different methods was found. For TH2 cells the correlation was less consistent. CONCLUSIONS: Probiotic bacteria have very different immune modulating capacities. Analysis of transcription factor mRNA is a suitable method for in vitro characterization of strains prior to clinical application. 2011 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
BACKGROUND & AIMS: Probiotic bacteria are used as food supplement in many different disease settings. The immune modulating capacity of different strains is not always properly tested which might result in a suboptimal choice of strains for clinical use. METHODS: The CD4 T cell responses to 19 different gut derived lactic acid bacteria were tested with different methods to show their diversity in immune modulation and to make a well-founded choice on which strains to use in future clinical trials. After co-culture of PBMC with bacteria, the induction of CD4(+) T cell subsets (regulatory T cells, T helper type (TH)1, TH2 and TH17) was analysed by rtPCR of transcription factor mRNA, intracellular FACS staining of transcription factors and cytokine production. RESULTS: Bacterial strains all have diverse, unique immune modulatory properties. Strains can induce Treg, TH1, TH2 and TH17 cells which can be shown at different levels of T cell activation, and is consistent for most strains tested. For TH1, TH17 and Treg, a positive correlation between the different methods was found. For TH2 cells the correlation was less consistent. CONCLUSIONS: Probiotic bacteria have very different immune modulating capacities. Analysis of transcription factor mRNA is a suitable method for in vitro characterization of strains prior to clinical application. 2011 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
Authors: Bin Zheng; Jeroen van Bergenhenegouwen; Saskia Overbeek; Hendrik J G van de Kant; Johan Garssen; Gert Folkerts; Paul Vos; Mary E Morgan; Aletta D Kraneveld Journal: PLoS One Date: 2014-05-02 Impact factor: 3.240
Authors: Amin N Olaimat; Iman Aolymat; Murad Al-Holy; Mutamed Ayyash; Mahmoud Abu Ghoush; Anas A Al-Nabulsi; Tareq Osaili; Vasso Apostolopoulos; Shao-Quan Liu; Nagendra P Shah Journal: NPJ Sci Food Date: 2020-10-05
Authors: Kimberley E Freedman; Jessica L Hill; Yuren Wei; Allegra R Vazquez; Diana S Grubb; Roxanne E Trotter; Scott D Wrigley; Sarah A Johnson; Michelle T Foster; Tiffany L Weir Journal: Int J Mol Sci Date: 2021-02-28 Impact factor: 5.923
Authors: Hamish Ck Angus; Paulo Cm Urbano; Gemma A Laws; Shijun Fan; Safina Gadeock; Michael Schultz; Grant Butt; Andrew J Highton; Roslyn A Kemp Journal: Clin Transl Immunology Date: 2022-08-01