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Literature DB >> 2167854

Purification and characterization of bovine brain type I phosphatidylinositol kinase.

Abstract

Investigation into the phosphatidylinositol kinase activities in bovine brain has revealed the presence of a type I PtdIns kinase activity. This classification is based upon potent inhibition by neutral detergent and the production of a phosphatidylinositol phosphate that can be distinguished from phosphatidyl-inositol-4-phosphate [PtdIns(4)P] by thin-layer chromatography. The enzyme has been substantially purified and the activity is associated with an 85-kDa polypeptide on SDS/polyacrylamide gel electrophoresis. Analysis of the product confirms the identification of the enzyme as a type I PtdIns kinase. The purified kinase has been characterized with respect to substrate dependence (Mg2+, ATP, PtdIns), substrate presentation (pure lipid versus mixed micelle) and specificity [PtdIns versus PtdIns(4)P and phosphatidylinositol 4,5-bisphosphate].

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Year: 1990 PMID： 2167854 DOI： 10.1111/j.1432-1033.1990.tb19185.x

Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956

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Cited

32 in total

1. Wortmannin blocks goldfish retinal phosphatidylinositol 3-kinase and neurite outgrowth.

Authors: Y Lavie; J Dybowski; B W Agranoff
Journal: Neurochem Res Date: 1997-04 Impact factor: 3.996

2. Purification and characterization of a phosphatidylinositol 3-kinase complex from bovine brain by using phosphopeptide affinity columns.

Authors: M J Fry; G Panayotou; R Dhand; F Ruiz-Larrea; I Gout; O Nguyen; S A Courtneidge; M D Waterfield
Journal: Biochem J Date: 1992-12-01 Impact factor: 3.857

3. SH2 domains of the p85 alpha subunit of phosphatidylinositol 3-kinase regulate binding to growth factor receptors.

Authors: C J McGlade; C Ellis; M Reedijk; D Anderson; G Mbamalu; A D Reith; G Panayotou; P End; A Bernstein; A Kazlauskas
Journal: Mol Cell Biol Date: 1992-03 Impact factor: 4.272

4. The C-terminal SH2 domain of p85 accounts for the high affinity and specificity of the binding of phosphatidylinositol 3-kinase to phosphorylated platelet-derived growth factor beta receptor.

Authors: A Klippel; J A Escobedo; W J Fantl; L T Williams
Journal: Mol Cell Biol Date: 1992-04 Impact factor: 4.272

5. Insulin-like growth factor and potassium depolarization maintain neuronal survival by distinct pathways: possible involvement of PI 3-kinase in IGF-1 signaling.

Authors: S R D'Mello; K Borodezt; S P Soltoff
Journal: J Neurosci Date: 1997-03-01 Impact factor: 6.167

10. Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system.

Authors: I Gout; R Dhand; G Panayotou; M J Fry; I Hiles; M Otsu; M D Waterfield
Journal: Biochem J Date: 1992-12-01 Impact factor: 3.857

Purification and characterization of bovine brain type I phosphatidylinositol kinase.

1. Wortmannin blocks goldfish retinal phosphatidylinositol 3-kinase and neurite outgrowth.

2. Purification and characterization of a phosphatidylinositol 3-kinase complex from bovine brain by using phosphopeptide affinity columns.

3. SH2 domains of the p85 alpha subunit of phosphatidylinositol 3-kinase regulate binding to growth factor receptors.

4. The C-terminal SH2 domain of p85 accounts for the high affinity and specificity of the binding of phosphatidylinositol 3-kinase to phosphorylated platelet-derived growth factor beta receptor.

5. Insulin-like growth factor and potassium depolarization maintain neuronal survival by distinct pathways: possible involvement of PI 3-kinase in IGF-1 signaling.

Review 6. PI3K: from the bench to the clinic and back.

7. The v-Src SH3 domain binds phosphatidylinositol 3'-kinase.

8. Purification and Characterization of a Soluble Phosphatidylinositol 4-Kinase from Carrot Suspension Culture Cells.

9. Inositide signalling in Chlamydomonas: characterization of a phosphatidylinositol 3-kinase gene.

10. Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system.