Literature DB >> 21676863

Arp2/3 complex is bound and activated by two WASP proteins.

Shae B Padrick1, Lynda K Doolittle, Chad A Brautigam, David S King, Michael K Rosen.   

Abstract

Actin related protein 2/actin related protein 3 (Arp2/3) complex nucleates new actin filaments in eukaryotic cells in response to signals from proteins in the Wiskott-Aldrich syndrome protein (WASP) family. The conserved VCA domain of WASP proteins activates Arp2/3 complex by inducing conformational changes and delivering the first actin monomer of the daughter filament. Previous models of activation have invoked a single VCA acting at a single site on Arp2/3 complex. Here we show that activation most likely involves engagement of two distinct sites on Arp2/3 complex by two VCA molecules, each delivering an actin monomer. One site is on Arp3 and the second is on ARPC1 and Arp2. The VCAs at these sites have distinct roles in activation. Our findings reconcile apparently conflicting literature on VCA activation of Arp2/3 complex and lead to a new model for this process.

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Year:  2011        PMID： 21676863      PMCID： PMC3158169          DOI： 10.1073/pnas.1100236108

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  42 in total

1.  Different WASP family proteins stimulate different Arp2/3 complex-dependent actin-nucleating activities.

Authors:  J Zalevsky; L Lempert; H Kranitz; R D Mullins
Journal:  Curr Biol       Date:  2001-12-11       Impact factor: 10.834

2.  Assembly of the bacteriophage T4 helicase: architecture and stoichiometry of the gp41-gp59 complex.

Authors:  Faoud T Ishmael; Stephen C Alley; Stephen J Benkovic
Journal:  J Biol Chem       Date:  2002-04-01       Impact factor: 5.157

3.  Activation of the Arp2/3 complex by the Listeria acta protein. Acta binds two actin monomers and three subunits of the Arp2/3 complex.

Authors:  J Zalevsky; I Grigorova; R D Mullins
Journal:  J Biol Chem       Date:  2000-10-11       Impact factor: 5.157

4.  Identification of functionally important residues of Arp2/3 complex by analysis of homology models from diverse species.

Authors:  Christopher C Beltzner; Thomas D Pollard
Journal:  J Mol Biol       Date:  2004-02-13       Impact factor: 5.469

Review 5.  Cellular control of actin nucleation.

Authors:  Matthew D Welch; R Dyche Mullins
Journal:  Annu Rev Cell Dev Biol       Date:  2002-04-02       Impact factor: 13.827

6.  The beta-thymosin/WH2 domain; structural basis for the switch from inhibition to promotion of actin assembly.

Authors:  Maud Hertzog; Carine van Heijenoort; Dominique Didry; Martin Gaudier; Jérôme Coutant; Benoît Gigant; Gérard Didelot; Thomas Préat; Marcel Knossow; Eric Guittet; Marie-France Carlier
Journal:  Cell       Date:  2004-05-28       Impact factor: 41.582

7.  Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complex.

Authors:  J B Marchand; D A Kaiser; T D Pollard; H N Higgs
Journal:  Nat Cell Biol       Date:  2001-01       Impact factor: 28.824

8.  Interaction of cortactin and N-WASp with Arp2/3 complex.

Authors:  Alissa M Weaver; John E Heuser; Andrei V Karginov; Wei-lih Lee; J Thomas Parsons; John A Cooper
Journal:  Curr Biol       Date:  2002-08-06       Impact factor: 10.834

9.  A conserved amphipathic helix in WASP/Scar proteins is essential for activation of Arp2/3 complex.

Authors:  Sanjay C Panchal; Donald A Kaiser; Eduardo Torres; Thomas D Pollard; Michael K Rosen
Journal:  Nat Struct Biol       Date:  2003-08

10.  Activation of Arp2/3 complex: addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2.

Authors:  Mark J Dayel; R Dyche Mullins
Journal:  PLoS Biol       Date:  2004-04-13       Impact factor: 8.029
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  115 in total

1.  Three-dimensional reconstructions of Arp2/3 complex with bound nucleation promoting factors.

Authors:  Xiao-Ping Xu; Isabelle Rouiller; Brian D Slaughter; Coumaran Egile; Eldar Kim; Jay R Unruh; Xiaoxue Fan; Thomas D Pollard; Rong Li; Dorit Hanein; Niels Volkmann
Journal:  EMBO J       Date:  2011-09-20       Impact factor: 11.598

2.  Actin filament curvature biases branching direction.

Authors:  Viviana I Risca; Evan B Wang; Ovijit Chaudhuri; Jia Jun Chia; Phillip L Geissler; Daniel A Fletcher
Journal:  Proc Natl Acad Sci U S A       Date:  2012-01-30       Impact factor: 11.205

3.  Isoform diversity in the Arp2/3 complex determines actin filament dynamics.

Authors:  Jasmine V G Abella; Chiara Galloni; Julien Pernier; David J Barry; Svend Kjær; Marie-France Carlier; Michael Way
Journal:  Nat Cell Biol       Date:  2015-12-14       Impact factor: 28.824

Review 4.  Myoblast fusion: lessons from flies and mice.

Authors:  Susan M Abmayr; Grace K Pavlath
Journal:  Development       Date:  2012-02       Impact factor: 6.868

5.  Three-color single molecule imaging shows WASP detachment from Arp2/3 complex triggers actin filament branch formation.

Authors:  Benjamin A Smith; Shae B Padrick; Lynda K Doolittle; Karen Daugherty-Clarke; Ivan R Corrêa; Ming-Qun Xu; Bruce L Goode; Michael K Rosen; Jeff Gelles
Journal:  Elife       Date:  2013-09-03       Impact factor: 8.140

6.  Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.

Authors:  Shih-Chieh Ti; Christopher T Jurgenson; Bradley J Nolen; Thomas D Pollard
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-15       Impact factor: 11.205

7.  Mycolactone Toxin Membrane Permeation: Atomistic versus Coarse-Grained MARTINI Simulations.

Authors:  Fikret Aydin; Rui Sun; Jessica M J Swanson
Journal:  Biophys J       Date:  2019-05-21       Impact factor: 4.033

8.  Arp2/3 Complex Is Required for Macrophage Integrin Functions but Is Dispensable for FcR Phagocytosis and In Vivo Motility.

Authors:  Jeremy D Rotty; Hailey E Brighton; Stephanie L Craig; Sreeja B Asokan; Ning Cheng; Jenny P Ting; James E Bear
Journal:  Dev Cell       Date:  2017-08-31       Impact factor: 12.270

9.  Three-dimensional reconstructions of actin filaments capped by Arp2/3 complex.

Authors:  Niels Volkmann; Christopher Page; Rong Li; Dorit Hanein
Journal:  Eur J Cell Biol       Date:  2014-01-25       Impact factor: 4.492

10.  Interactions of isolated C-terminal fragments of neural Wiskott-Aldrich syndrome protein (N-WASP) with actin and Arp2/3 complex.

Authors:  Jean-François Gaucher; Chloé Maugé; Dominique Didry; Bérengère Guichard; Louis Renault; Marie-France Carlier
Journal:  J Biol Chem       Date:  2012-07-30       Impact factor: 5.157
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