Literature DB >> 2167630

Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27.

Y Koyama1, S Okamoto, K Furukawa.   

Abstract

The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid of Thermus cryptic plasmid pTT8, and beta-galactosidases were expressed constitutively.

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Year:  1990        PMID： 2167630      PMCID： PMC184593          DOI： 10.1128/aem.56.7.2251-2254.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

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Authors:  T J Silhavy; J R Beckwith
Journal:  Microbiol Rev       Date:  1985-12

2.  Physical characterization of a plasmid (pTT1) isolated from Thermus thermophilus.

Authors:  M D Eberhard; C Vásquez; P Valenzuela; R Vicuña; A Yudelevich
Journal:  Plasmid       Date:  1981-07       Impact factor: 3.466

3.  Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing.

Authors:  D R Russell; G N Bennett
Journal:  Gene       Date:  1982-12       Impact factor: 3.688

4.  Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.

Authors:  M J Casadaban; S N Cohen
Journal:  J Mol Biol       Date:  1980-04       Impact factor: 5.469

5.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

6.  Induction and characterization of -galactosidase in an extreme thermophile.

Authors:  J T Ulrich; G A McFeters; K L Temple
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

7.  Comparative study of isoenzyme formation of bacterial beta-galactosidase.

Authors:  R P Erickson; E Steers
Journal:  J Bacteriol       Date:  1970-04       Impact factor: 3.490

8.  Isolation of extrachromosomal deoxyribonucleic acids from extremely thermophilic bacteria.

Authors:  F Hishinuma; T Tanaka; K Sakaguchi
Journal:  J Gen Microbiol       Date:  1978-02
  8 in total
  21 in total

1.  Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli.

Authors:  M Ishiguro; S Kaneko; A Kuno; Y Koyama; S Yoshida; G G Park; Y Sakakibara; I Kusakabe; H Kobayashi
Journal:  Appl Environ Microbiol       Date:  2001-04       Impact factor: 4.792

2.  Curing the Megaplasmid pTT27 from Thermus thermophilus HB27 and Maintaining Exogenous Plasmids in the Plasmid-Free Strain.

Authors:  Naoto Ohtani; Masaru Tomita; Mitsuhiro Itaya
Journal:  Appl Environ Microbiol       Date:  2015-12-28       Impact factor: 4.792

3.  Recombination-Deficient Mutants of an Extreme Thermophile, Thermus thermophilus.

Authors:  T Takada; S Akanuma; T Kotsuka; M Tamakoshi; A Yamagishi; T Oshima
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

4.  Structure of the beta-galactosidase gene from Thermus sp. strain T2: expression in Escherichia coli and purification in a single step of an active fusion protein.

Authors:  A Vian; A V Carrascosa; J L García; E Cortés
Journal:  Appl Environ Microbiol       Date:  1998-06       Impact factor: 4.792

5.  Characterization of the melA locus for alpha-galactosidase in Lactobacillus plantarum.

Authors:  Aurelio Silvestroni; Cristelle Connes; Fernando Sesma; Graciela Savoy De Giori; Jean-Christophe Piard
Journal:  Appl Environ Microbiol       Date:  2002-11       Impact factor: 4.792

6.  Cloning and expression of a beta-glycosidase gene from Thermus thermophilus. Sequence and biochemical characterization of the encoded enzyme.

Authors:  M Dion; L Fourage; J N Hallet; B Colas
Journal:  Glycoconj J       Date:  1999-01       Impact factor: 2.916

7.  Production of recombinant alpha-galactosidases in Thermus thermophilus.

Authors:  O Fridjonsson; R Mattes
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

8.  Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.

Authors:  Lei Wang; Jana Hoffmann; Hildegard Watzlawick; Josef Altenbuchner
Journal:  Appl Environ Microbiol       Date:  2015-12-11       Impact factor: 4.792

9.  Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase.

Authors:  Ho-Shin Park; Kevin J Kayser; Jung-Ho Kwak; John J Kilbane
Journal:  J Ind Microbiol Biotechnol       Date:  2004-05-12       Impact factor: 3.346

10.  A reporter gene system for the precise measurement of promoter activity in Thermus thermophilus HB27.

Authors:  Atsushi Fujita; Takaaki Sato; Yoshinori Koyama; Yoshio Misumi
Journal:  Extremophiles       Date:  2015-09-23       Impact factor: 2.395
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