Literature DB >> 21675793

Fluorescent liposomes for differential interactions with glycosaminoglycans.

Erin K Nyren-Erickson1, Manas K Haldar, Yan Gu, Steven Y Qian, Daniel L Friesner, Sanku Mallik.   

Abstract

We have successfully synthesized a lipid containing the pyranine dye as the hydrophilic headgroup. This lipid was incorporated into liposomes with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine as the major component. The resultant liposomes displayed differential modulations in fluorescence emission intensity in the presence of nanomolar concentrations of different glycosaminoglycans. Linear discriminant analysis of the fluorescence response data demonstrate that the liposomes are able to distinguish between different GAGs. In addition, we also demonstrate that the liposomes incorporating the pyranine lipid are able to distinguish between dilute serum from healthy individuals and serum containing elevated chondroitin sulfate (simulated serum from an Alzheimer's disease patient).

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Year:  2011        PMID: 21675793      PMCID: PMC3146584          DOI: 10.1021/ac2009993

Source DB:  PubMed          Journal:  Anal Chem        ISSN: 0003-2700            Impact factor:   6.986


  19 in total

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  4 in total

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4.  Glycosaminoglycan-mediated selective changes in the aggregation states, zeta potentials, and intrinsic stability of liposomes.

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  4 in total

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