| Literature DB >> 2167557 |
M E Boursnell1, P F Green, A C Samson, J I Campbell, A Deuter, R W Peters, N S Millar, P T Emmerson, M M Binns.
Abstract
The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fowlpox virus. Expression was demonstrated in tissue culture, a protein of the correct size for fully glycosylated HN protein being recognized by an HN-specific monoclonal antibody on Western blots. When the recombinant fowlpox virus was inoculated into chickens by intravenous or wing-web routes, antibody which recognizes HN from purified NDV virions was produced. Protective immunity to NDV was generated in the chickens; at the highest dose of vaccine 100% of the chickens tested were protected against challenge with a virulent strain of NDV.Entities:
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Year: 1990 PMID： 2167557 DOI： 10.1016/0042-6822(90)90408-j
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616