Hana Tuby1, Lidya Maltz, Uri Oron. 1. Department of Zoology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel.
Abstract
BACKGROUND AND OBJECTIVES: The adult mammalian heart is known to have a very limited regenerative capacity following acute ischemia. In this study we investigated the hypothesis that photobiostimulation of autologous bone-marrow-derived mesenchymal stem cells (MSCs) by low-level laser therapy (LLLT) applied to the bone marrow (BM), may migrate to the infarcted area and thus attenuate the scarring processes following myocardial infarction (MI). MATERIALS AND METHODS: Sprague-Dawley rats underwent experimental MI. LLLT (Ga-Al-As diode laser, power density 10 mW/cm², for 100 seconds) was then applied to the BM of the exposed tibia at different time intervals post-MI (20 minutes and 4 hours). Sham-operated infarcted rats served as control. RESULTS: Infarct size and ventricular dilatation were significantly reduced (76% and 75%, respectively) in the laser-treated rats 20 minutes post-MI as compared to the control-non-treated rats at 3 weeks post-MI. There was also a significant 25-fold increase in cell density of c-kit+ cells in the infarcted area of the laser-treated rats (20 minutes post-MI) as compared to the non-laser-treated controls. CONCLUSION: The application of LLLT to autologous BM of rats post-MI offers a novel approach to induce BM-derived MSCs, which are consequently recruited from the circulation to the infarcted heart and markedly attenuate the scarring process post-MI.
BACKGROUND AND OBJECTIVES: The adult mammalian heart is known to have a very limited regenerative capacity following acute ischemia. In this study we investigated the hypothesis that photobiostimulation of autologous bone-marrow-derived mesenchymal stem cells (MSCs) by low-level laser therapy (LLLT) applied to the bone marrow (BM), may migrate to the infarcted area and thus attenuate the scarring processes following myocardial infarction (MI). MATERIALS AND METHODS:Sprague-Dawley rats underwent experimental MI. LLLT (Ga-Al-As diode laser, power density 10 mW/cm², for 100 seconds) was then applied to the BM of the exposed tibia at different time intervals post-MI (20 minutes and 4 hours). Sham-operated infarctedrats served as control. RESULTS: Infarct size and ventricular dilatation were significantly reduced (76% and 75%, respectively) in the laser-treated rats 20 minutes post-MI as compared to the control-non-treated rats at 3 weeks post-MI. There was also a significant 25-fold increase in cell density of c-kit+ cells in the infarcted area of the laser-treated rats (20 minutes post-MI) as compared to the non-laser-treated controls. CONCLUSION: The application of LLLT to autologous BM of rats post-MI offers a novel approach to induce BM-derived MSCs, which are consequently recruited from the circulation to the infarcted heart and markedly attenuate the scarring process post-MI.
Authors: Tanupriya Agrawal; Gaurav K Gupta; Vikrant Rai; James D Carroll; Michael R Hamblin Journal: Dose Response Date: 2014-09-22 Impact factor: 2.658
Authors: A S Chernov; D A Reshetnikov; G K Ristsov; Yu A Kovalitskaya; A M Ermakov; A A Manokhin; A V Simakin; R G Vasilov; S V Gudkov Journal: J Biol Phys Date: 2019-10-08 Impact factor: 1.365
Authors: Pinar Avci; Theodore T Nyame; Gaurav K Gupta; Magesh Sadasivam; Michael R Hamblin Journal: Lasers Surg Med Date: 2013-06-07 Impact factor: 4.025
Authors: Praveen R Arany; Andrew Cho; Tristan D Hunt; Gursimran Sidhu; Kyungsup Shin; Eason Hahm; George X Huang; James Weaver; Aaron Chih-Hao Chen; Bonnie L Padwa; Michael R Hamblin; Mary Helen Barcellos-Hoff; Ashok B Kulkarni; David J Mooney Journal: Sci Transl Med Date: 2014-05-28 Impact factor: 17.956