Literature DB >> 2167341

Differentiation of cytomegalovirus strains by restriction analysis of DNA sequences amplified from clinical specimens.

S W Chou1.   

Abstract

DNA sequences of 2.0 to 2.7 kb were amplified by polymerase chain reaction from four genes (major immediate-early, DNA polymerase, and glycoproteins B and H) of 20 distinct strains of cytomegalovirus (CMV). Analysis of the unpurified reaction products using selected restriction enzymes showed sufficient diversity of profiles among strains to differentiate all 20. Sequences suitable for restriction analysis were also amplified directly from clinical urine specimens and showed restriction profiles comparable to those of sequences amplified from corresponding CMV culture isolates. Ability to identify specific strains of CMV in amplification products facilitates molecular epidemiologic studies.

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Year:  1990        PMID： 2167341     DOI： 10.1093/infdis/162.3.738

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   5.226


  21 in total

1.  Effect of interstrain variation on diagnostic DNA amplification of the cytomegalovirus major immediate-early gene region.

Authors:  S Chou
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Rapid epidemiologic analysis of cytomegalovirus by using polymerase chain reaction amplification of the L-S junction region.

Authors:  D M Sokol; G J Demmler; G J Buffone
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

3.  Comparative sequence analysis of human cytomegalovirus strains.

Authors:  R Lehner; T Stamminger; M Mach
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

4.  Cytomegalovirus infection in newborns and their family members: polymerase chain reaction analysis of isolates.

Authors:  I E Souza; A Gregg; D Pfab; J D Dawson; P Benson; M E O'Neill; J R Murph; S J Petheram; J F Bale
Journal:  Infection       Date:  1997 May-Jun       Impact factor: 3.553

5.  A polymorphic region of the human cytomegalovirus genome encoding putative glycoproteins.

Authors:  S Watanabe; M Shinkai; S Hitomi; H Kozuka; S Kimura; K Shimada; R Hondo; N Yamaguchi
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

6.  Strain-dependent differences in the human cytomegalovirus replication origin.

Authors:  Z Chen; S Watanabe; N Yamaguchi
Journal:  Arch Virol       Date:  1996       Impact factor: 2.574

7.  Characterization of human cytomegalovirus strains by analysis of short tandem repeat polymorphisms.

Authors:  A Walker; S J Petheram; L Ballard; J R Murph; G J Demmler; J F Bale
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

8.  Different cytomegalovirus glycoprotein B genotype distribution in serum and cerebrospinal fluid specimens determined by a novel multiplex nested PCR.

Authors:  David Tarragó; Carmen Quereda; Antonio Tenorio
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

9.  Ganciclovir sensitivity of cytomegalovirus at diagnosis and during treatment of cytomegalovirus pneumonia in marrow transplant recipients.

Authors:  M A Slavin; R R Bindra; C A Gleaves; M B Pettinger; R A Bowden
Journal:  Antimicrob Agents Chemother       Date:  1993-06       Impact factor: 5.191

10.  Comparative analysis of human cytomegalovirus a-sequence in multiple clinical isolates by using polymerase chain reaction and restriction fragment length polymorphism assays.

Authors:  J A Zaia; G Gallez-Hawkins; M A Churchill; A Morton-Blackshere; H Pande; S P Adler; G M Schmidt; S J Forman
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948
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