J Qiu1, H Li, H Meng, C Hu, J Li, M Luo, J Dong, X Wang, J Wang, Y Deng, X Deng. 1. Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
Abstract
AIMS: To investigate the influence of subinhibitory concentrations of luteolin on the production of α-toxin in Staphylococcus aureus. METHODS AND RESULTS: The minimal inhibitory concentrations (MICs) were determined using a broth microdilution method, and the MICs of luteolin against the tested Staph. aureus strains ranged from 16 to 64 μg ml(-1). Haemolysis, Western blot and real-time reverse transcription (RT)-PCR assays were used to evaluate the effect of luteolin on Staph. aureusα-toxin secretion and on the level of gene expression, respectively. The data indicated that subinhibitory concentrations of luteolin dose dependently decreased the production of α-toxin in both meticillin-sensitive Staph. aureus (MSSA) and meticillin-resistant Staph. aureus (MRSA). Furthermore, the transcriptional levels of agr (accessory gene regulator) in Staph. aureus were also inhibited by luteolin. CONCLUSIONS: Luteolin decreases the production and/or secretion of α-toxin in Staph. aureus; the reduced production may be dependent, in part, upon the luteolin-induced inhibition of the agr locus. SIGNIFICANCE AND IMPACT OF THE STUDY: The findings indicate that luteolin may be used as a basis for the development of antimicrobial agents aimed at bacterial virulence factors.
AIMS: To investigate the influence of subinhibitory concentrations of luteolin on the production of α-toxin in Staphylococcus aureus. METHODS AND RESULTS: The minimal inhibitory concentrations (MICs) were determined using a broth microdilution method, and the MICs of luteolin against the tested Staph. aureus strains ranged from 16 to 64 μg ml(-1). Haemolysis, Western blot and real-time reverse transcription (RT)-PCR assays were used to evaluate the effect of luteolin on Staph. aureusα-toxin secretion and on the level of gene expression, respectively. The data indicated that subinhibitory concentrations of luteolin dose dependently decreased the production of α-toxin in both meticillin-sensitive Staph. aureus (MSSA) and meticillin-resistant Staph. aureus (MRSA). Furthermore, the transcriptional levels of agr (accessory gene regulator) in Staph. aureus were also inhibited by luteolin. CONCLUSIONS: Luteolin decreases the production and/or secretion of α-toxin in Staph. aureus; the reduced production may be dependent, in part, upon the luteolin-induced inhibition of the agr locus. SIGNIFICANCE AND IMPACT OF THE STUDY: The findings indicate that luteolin may be used as a basis for the development of antimicrobial agents aimed at bacterial virulence factors.
Authors: Muhammad Usman Amin; Muhammad Khurram; Taj Ali Khan; Hani S Faidah; Zia Ullah Shah; Shafiq Ur Rahman; Abdul Haseeb; Muhammad Ilyas; Naseem Ullah; Sahibzada Muhammad Umar Khayam; Marcello Iriti Journal: Int J Mol Sci Date: 2016-11-22 Impact factor: 5.923