| Literature DB >> 2167177 |
T Okamoto1, T Katada, Y Murayama, M Ui, E Ogata, I Nishimoto.
Abstract
The insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/man6PR) can directly interact with and activate Gi-2, a GTP binding protein (G protein). We found that the segment of residues 2410-2423 in the human IGF-II/man6PR activates Gi-2 in a manner similar to G-coupled receptors. We observed a hierarchy of the segment action when tested on various G proteins, with an order of Gi-2 greater than Gi-1 approximately Gi-3 greater than Go. The segment had no effect on Gs or low molecular weight G proteins. The segment action depended on its primary structure and was potentiated when the segment was connected with a part of the receptor transmembrane region. Finally, the Gi-2-activating function of the human IGF-II/man6PR could be blocked by an antibody against the segment, indicating a critical role for this small region of the receptor.Entities:
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Year: 1990 PMID： 2167177 DOI： 10.1016/0092-8674(90)90116-v
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582