| Literature DB >> 21671178 |
Yuhui Li1,2, Hongxia Zhou1, Enming Xing1, Meera Dassarath1,3, Jinghua Ren1, Xiaorong Dong1, Hongli Liu1, Kunyu Yang4, Gang Wu1.
Abstract
The present study investigated the enhanced radiosensitivity of U-251 cells induced by sodium butyrate (NaB) and its possible mechanisms. Increased radiosensitivity of U251 cells was examined by clonogenic cell survival assays. The expression of Ku70 mRNA and protein was detected by using RT-PCR and Western blotting respectively. γ-H2AX foci were measured at different time points after ionizing irradiation alone or combined with NaB treatment. The results showed that cell survival rate was significantly reduced, both D0 and Dq values were decreased (D0: 1.43 Gy vs. 1.76 Gy; Dq: 1.22 Gy vs. 2.05 Gy) after the combined treatment as compared with irradiation alone, and sensitivity enhancing ratio (SER) reached 1.23. The average number of γ-H2AX foci per cell receiving the combined treatment was significantly increased at different time points, and the expression levels of Ku70 mRNA and protein were suppressed by NaB in a dose-dependent manner. It was concluded that enhanced radiosensitivity induced by NaB involves an inhibited expression of Ku70 and an increase in γ-H2AX foci, which suggests decreased ability in DSB repair.Entities:
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Year: 2011 PMID： 21671178 DOI： 10.1007/s11596-011-0381-8
Source DB: PubMed Journal: J Huazhong Univ Sci Technolog Med Sci ISSN： 1672-0733