Literature DB >> 21670516

Cloning and expression of a β-galactosidase gene of Bacillus circulans.

Jingyuan Song1, Hiroyuki Imanaka, Koreyoshi Imamura, Masashi Minoda, Toru Katase, Yukiko Hoshi, Shotaro Yamaguchi, Kazuhiro Nakanishi.   

Abstract

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

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Year:  2011        PMID： 21670516     DOI： 10.1271/bbb.110014

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  8 in total

1.  Monobody-mediated alteration of enzyme specificity.

Authors:  Shun-Ichi Tanaka; Tetsuya Takahashi; Akiko Koide; Satoru Ishihara; Satoshi Koikeda; Shohei Koide
Journal:  Nat Chem Biol       Date:  2015-08-31       Impact factor: 15.040

2.  β-galactosidase stability at high substrate concentrations.

Authors:  Anja Warmerdam; Remko M Boom; Anja Em Janssen
Journal:  Springerplus       Date:  2013-08-27

3.  Biochemical characterization of mutants in the active site residues of the β-galactosidase enzyme of Bacillus circulans ATCC 31382.

Authors:  Jelle B Bultema; Bas J H Kuipers; Lubbert Dijkhuizen
Journal:  FEBS Open Bio       Date:  2014-11-12       Impact factor: 2.693

4.  Analysis of Domain Architecture and Phylogenetics of Family 2 Glycoside Hydrolases (GH2).

Authors:  David Talens-Perales; Anna Górska; Daniel H Huson; Julio Polaina; Julia Marín-Navarro
Journal:  PLoS One       Date:  2016-12-08       Impact factor: 3.240

5.  Biochemical Characterization of the Functional Roles of Residues in the Active Site of the β-Galactosidase from Bacillus circulans ATCC 31382.

Authors:  Huifang Yin; Tjaard Pijning; Xiangfeng Meng; Lubbert Dijkhuizen; Sander S van Leeuwen
Journal:  Biochemistry       Date:  2017-06-05       Impact factor: 3.162

6.  Activation of LacZ gene in Escherichia coli DH5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations.

Authors:  Ahmed A Hamed; Mohamed Khedr; Mohamed Abdelraof
Journal:  J Genet Eng Biotechnol       Date:  2020-12-02

7.  Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides.

Authors:  Pavlína Nekvasilová; Michaela Hovorková; Zuzana Mészáros; Lucie Petrásková; Helena Pelantová; Vladimír Křen; Kristýna Slámová; Pavla Bojarová
Journal:  Int J Mol Sci       Date:  2022-04-07       Impact factor: 6.208

8.  A New β-Galactosidase from the Antarctic Bacterium Alteromonas sp. ANT48 and Its Potential in Formation of Prebiotic Galacto-Oligosaccharides.

Authors:  Shangyong Li; Xiangjie Zhu; Mengxin Xing
Journal:  Mar Drugs       Date:  2019-10-23       Impact factor: 5.118
  8 in total

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