S Panzer1, P Jilma. 1. Department for Blood Group Serology and Transfusion Medicine, Medical University Vienna, Vienna, Austria. simon.panzer@meduniwien.ac.at
Abstract
BACKGROUND: Currently only indirect measures are required for monitoring the function of platelets in platelet concentrates (PC). METHODS: This is an overview on currently available commercialized methods that have been used to determine platelet function in donors, concentrates and after transfusion. We show examples for the application of the no/low shear methods light-transmission aggregometry, flow cytometry, multiple electrode aggregometry, thrombelastography and dynamic light scattering, and those applying high shear, the platelet function analyzer-100, and the cone and plate analyzer. Advantages and disadvantages of the various methods to screen donors, evaluate the haemostatic properties maintained in the PC and after transfusion are discussed, based on considerations of platelet physiology, and the feasibility of the various procedures. This survey focuses on reports from the last 10 years, as the technology for the production of PCs has advanced significantly during the last few years. CONCLUSION: Specific aspects of platelet function can be assessed by the no/low shear methods, while the high shear methods provide more general analysis of platelet haemostatic competence. Yet, there is no strong evidence that the in vitro data correspond with the clinical outcome.
BACKGROUND: Currently only indirect measures are required for monitoring the function of platelets in platelet concentrates (PC). METHODS: This is an overview on currently available commercialized methods that have been used to determine platelet function in donors, concentrates and after transfusion. We show examples for the application of the no/low shear methods light-transmission aggregometry, flow cytometry, multiple electrode aggregometry, thrombelastography and dynamic light scattering, and those applying high shear, the platelet function analyzer-100, and the cone and plate analyzer. Advantages and disadvantages of the various methods to screen donors, evaluate the haemostatic properties maintained in the PC and after transfusion are discussed, based on considerations of platelet physiology, and the feasibility of the various procedures. This survey focuses on reports from the last 10 years, as the technology for the production of PCs has advanced significantly during the last few years. CONCLUSION: Specific aspects of platelet function can be assessed by the no/low shear methods, while the high shear methods provide more general analysis of platelet haemostatic competence. Yet, there is no strong evidence that the in vitro data correspond with the clinical outcome.
Authors: Alex Gatt; Fabian Bonello; Raphael Buttigieg; Samuel Debono; Patricia Brincat; Charlie Grima; Peter Gatt; Thomas Lofaro; Stefan Laspina Journal: Blood Transfus Date: 2014-06-05 Impact factor: 3.443
Authors: Reinaldo Barros Geraldo; Plínio Cunha Sathler; André Luiz Lourenço; Max Seidy Saito; Lucio M Cabral; Pabulo Henrique Rampelotto; Helena Carla Castro Journal: Int J Mol Sci Date: 2014-10-07 Impact factor: 5.923