OBJECTIVE: To measure levels of 8-isoprostane and Lipoxin A4 in the exhaled breath condensate of children (7-17 yrs old) recovering from status asthmaticus in a pediatric intensive care unit and to compare their respective levels in the exhaled breath condensate collected from age-matched "healthy" children enrolled from an ambulatory pediatric clinic during well-child visits. DESIGN: Prospective case-controlled study. SETTING: Teaching hospitals and a research laboratory. PATIENTS: Children recovering from status asthmaticus and age-matched controls. INTERVENTIONS: Collection of exhaled breath condensate from patients recovering from status asthmaticus and controls for purpose of measurement of 8-isoprostane and Lipoxin A4. MEASUREMENTS AND MAIN RESULTS: There was no difference in age (11.9 ± 3.0 vs. 12.0 ± 3.3 yrs, p = .9) between patients and control subjects. All participants completed the exhaled breath condensate collection without complications. There was no difference in the pulmonary index (3.3 ± 2.2 vs. 3.1 ± 1.9, p = 1.0) after collection of exhaled breath condensate compared with baseline values in patients with status asthmaticus. The level of 8-isoprostane was significantly higher (63 ± 9 vs. 41 ± 13 pg/mL, p < .001), whereas the level of Lipoxin A4 was significantly lower (5.6 ± 2.9 vs. 10.5 ± 3.1 ng/mL, p < .001) in the exhaled breath condensate from children recovering from status asthmaticus compared with control subjects. CONCLUSIONS: 8-Isoprostane was elevated and Lipoxin A4 is decreased in the exhaled breath condensate of children recovering from status asthmaticus in a pediatric intensive care unit. These data may provide new insight into the pathophysiology of asthma in children in this clinical setting.
OBJECTIVE: To measure levels of 8-isoprostane and Lipoxin A4 in the exhaled breath condensate of children (7-17 yrs old) recovering from status asthmaticus in a pediatric intensive care unit and to compare their respective levels in the exhaled breath condensate collected from age-matched "healthy" children enrolled from an ambulatory pediatric clinic during well-child visits. DESIGN: Prospective case-controlled study. SETTING: Teaching hospitals and a research laboratory. PATIENTS: Children recovering from status asthmaticus and age-matched controls. INTERVENTIONS: Collection of exhaled breath condensate from patients recovering from status asthmaticus and controls for purpose of measurement of 8-isoprostane and Lipoxin A4. MEASUREMENTS AND MAIN RESULTS: There was no difference in age (11.9 ± 3.0 vs. 12.0 ± 3.3 yrs, p = .9) between patients and control subjects. All participants completed the exhaled breath condensate collection without complications. There was no difference in the pulmonary index (3.3 ± 2.2 vs. 3.1 ± 1.9, p = 1.0) after collection of exhaled breath condensate compared with baseline values in patients with status asthmaticus. The level of 8-isoprostane was significantly higher (63 ± 9 vs. 41 ± 13 pg/mL, p < .001), whereas the level of Lipoxin A4 was significantly lower (5.6 ± 2.9 vs. 10.5 ± 3.1 ng/mL, p < .001) in the exhaled breath condensate from children recovering from status asthmaticus compared with control subjects. CONCLUSIONS:8-Isoprostane was elevated and Lipoxin A4 is decreased in the exhaled breath condensate of children recovering from status asthmaticus in a pediatric intensive care unit. These data may provide new insight into the pathophysiology of asthma in children in this clinical setting.
Authors: M Sanak; B D Levy; C B Clish; N Chiang; K Gronert; L Mastalerz; C N Serhan; A Szczeklik Journal: Eur Respir J Date: 2000-07 Impact factor: 16.671
Authors: Levente József; Christine Zouki; Nicos A Petasis; Charles N Serhan; János G Filep Journal: Proc Natl Acad Sci U S A Date: 2002-09-16 Impact factor: 11.205
Authors: Bruce D Levy; George T De Sanctis; Pallavi R Devchand; Eugene Kim; Kate Ackerman; Birgitta A Schmidt; Wojciech Szczeklik; Jeffrey M Drazen; Charles N Serhan Journal: Nat Med Date: 2002-08-12 Impact factor: 53.440
Authors: E Baraldi; S Carraro; R Alinovi; A Pesci; L Ghiro; A Bodini; G Piacentini; F Zacchello; S Zanconato Journal: Thorax Date: 2003-06 Impact factor: 9.139
Authors: Rosalia Gagliardo; Pascal Chanez; Marc Mathieu; Andreina Bruno; Giorgia Costanzo; Claire Gougat; Isabelle Vachier; Jean Bousquet; Giovanni Bonsignore; Antonio M Vignola Journal: Am J Respir Crit Care Med Date: 2003-07-31 Impact factor: 21.405
Authors: J F Hunt; K Fang; R Malik; A Snyder; N Malhotra; T A Platts-Mills; B Gaston Journal: Am J Respir Crit Care Med Date: 2000-03 Impact factor: 21.405
Authors: Shamsah Kazani; Anna Planaguma; Emiko Ono; Matteo Bonini; Muhammad Zahid; Gautham Marigowda; Michael E Wechsler; Bruce D Levy; Elliot Israel Journal: J Allergy Clin Immunol Date: 2013-04-19 Impact factor: 10.793
Authors: Rafael I Jaén; Sergio Sánchez-García; María Fernández-Velasco; Lisardo Boscá; Patricia Prieto Journal: Front Immunol Date: 2021-04-23 Impact factor: 7.561