Literature DB >> 21666018

Development of a method for markerless gene deletion in Pseudomonas putida.

Nadja Graf1, Josef Altenbuchner.   

Abstract

We developed a negative counterselection system for Pseudomonas putida based on uracil phosphoribosyltransferase (UPRTase) and sensitivity against the antimetabolite 5-fluorouracil (5-FU). We constructed a P. putida strain that is resistant to 5-FU and constructed vectors for the deletion of the surface adhesion protein gene, the flagellum biosynthesis operon, and two endonuclease genes. The genes were efficiently disrupted and left a markerless chromosomal in-frame deletion.

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Year:  2011        PMID： 21666018      PMCID： PMC3147467          DOI： 10.1128/AEM.05055-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria.

Authors:  Christopher J Marx; Mary E Lidstrom
Journal:  Biotechniques       Date:  2002-11       Impact factor: 1.993

2.  Positive selection vectors based on palindromic DNA sequences.

Authors:  J Altenbuchner; P Viell; I Pelletier
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

3.  Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.

Authors:  G BERTANI
Journal:  J Bacteriol       Date:  1951-09       Impact factor: 3.490

Review 4.  Counterselectable markers: untapped tools for bacterial genetics and pathogenesis.

Authors:  J M Reyrat; V Pelicic; B Gicquel; R Rappuoli
Journal:  Infect Immun       Date:  1998-09       Impact factor: 3.441

5.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

6.  Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.

Authors:  K E Nelson; C Weinel; I T Paulsen; R J Dodson; H Hilbert; V A P Martins dos Santos; D E Fouts; S R Gill; M Pop; M Holmes; L Brinkac; M Beanan; R T DeBoy; S Daugherty; J Kolonay; R Madupu; W Nelson; O White; J Peterson; H Khouri; I Hance; P Chris Lee; E Holtzapple; D Scanlan; K Tran; A Moazzez; T Utterback; M Rizzo; K Lee; D Kosack; D Moestl; H Wedler; J Lauber; D Stjepandic; J Hoheisel; M Straetz; S Heim; C Kiewitz; J A Eisen; K N Timmis; A Düsterhöft; B Tümmler; C M Fraser
Journal:  Environ Microbiol       Date:  2002-12       Impact factor: 5.491

7.  Development of a markerless genetic exchange system for Desulfovibrio vulgaris Hildenborough and its use in generating a strain with increased transformation efficiency.

Authors:  Kimberly L Keller; Kelly S Bender; Judy D Wall
Journal:  Appl Environ Microbiol       Date:  2009-10-16       Impact factor: 4.792

8.  A new mutation delivery system for genome-scale approaches in Bacillus subtilis.

Authors:  Céline Fabret; S Dusko Ehrlich; Philippe Noirot
Journal:  Mol Microbiol       Date:  2002-10       Impact factor: 3.501

9.  A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.

Authors:  T T Hoang; R R Karkhoff-Schweizer; A J Kutchma; H P Schweizer
Journal:  Gene       Date:  1998-05-28       Impact factor: 3.688

10.  Development and application of a upp-based counterselective gene replacement system for the study of the S-layer protein SlpX of Lactobacillus acidophilus NCFM.

Authors:  Yong Jun Goh; M Andrea Azcárate-Peril; Sarah O'Flaherty; Evelyn Durmaz; Florence Valence; Julien Jardin; Sylvie Lortal; Todd R Klaenhammer
Journal:  Appl Environ Microbiol       Date:  2009-03-20       Impact factor: 4.792
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  23 in total

Review 1.  Leveraging synthetic biology for producing bioactive polyketides and non-ribosomal peptides in bacterial heterologous hosts.

Authors:  Taylor B Cook; Brian F Pfleger
Journal:  Medchemcomm       Date:  2019-04-25       Impact factor: 3.597

2.  Ethylene glycol metabolism by Pseudomonas putida.

Authors:  Björn Mückschel; Oliver Simon; Janosch Klebensberger; Nadja Graf; Bettina Rosche; Josef Altenbuchner; Jens Pfannstiel; Armin Huber; Bernhard Hauer
Journal:  Appl Environ Microbiol       Date:  2012-09-28       Impact factor: 4.792

3.  Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.

Authors:  Taylor B Cook; Jacqueline M Rand; Wasti Nurani; Dylan K Courtney; Sophia A Liu; Brian F Pfleger
Journal:  J Ind Microbiol Biotechnol       Date:  2018-01-03       Impact factor: 3.346

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Authors:  Changyi Zhang; Qunxin She; Hongkai Bi; Rachel J Whitaker
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5.  Development of a Markerless Deletion Mutagenesis System in Nitrate-Reducing Bacterium Rhodanobacter denitrificans.

Authors:  Xuanyu Tao; Aifen Zhou; Megan L Kempher; Jiantao Liu; Mu Peng; Yuan Li; Jonathan P Michael; Romy Chakraborty; Adam M Deutschbauer; Adam P Arkin; Jizhong Zhou
Journal:  Appl Environ Microbiol       Date:  2022-06-23       Impact factor: 5.005

6.  Chromosomal insertions in the Lactobacillus casei upp gene that are useful for vaccine expression.

Authors:  Bai-fen Song; Long-zhu Ju; Yi-jing Li; Li-jie Tang
Journal:  Appl Environ Microbiol       Date:  2014-03-21       Impact factor: 4.792

7.  Functional Characterization of the Mannitol Promoter of Pseudomonas fluorescens DSM 50106 and Its Application for a Mannitol-Inducible Expression System for Pseudomonas putida KT2440.

Authors:  Jana Hoffmann; Josef Altenbuchner
Journal:  PLoS One       Date:  2015-07-24       Impact factor: 3.240

8.  Engineering Pseudomonas putida KT2440 for simultaneous degradation of carbofuran and chlorpyrifos.

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Journal:  Microb Biotechnol       Date:  2016-07-15       Impact factor: 5.813

Review 9.  Bacterial cellular engineering by genome editing and gene silencing.

Authors:  Nobutaka Nakashima; Kentaro Miyazaki
Journal:  Int J Mol Sci       Date:  2014-02-18       Impact factor: 5.923

10.  Stepwise genetic engineering of Pseudomonas putida enables robust heterologous production of prodigiosin and glidobactin A.

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Journal:  Metab Eng       Date:  2021-06-24       Impact factor: 8.829
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