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Literature DB >> 216633

Rapid micromeasurement of neutrophil exocytosis.

Abstract

A simple method is described for the visual detection of neutrophil granule exocytosis. The microassay measures release of myeloperoxidase from azurophilic granules following neutrophil stimulation and can be accomplished in less than 30 min. The assay is sensitive, reproducible from day to day, and does not require separation of leukocytes from the reaction before enzyme assay. The procedure is also applicable to the rapid measurement of the suppression of degranulation by antiinflammatory agents.

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Year: 1978 PMID： 216633 DOI： 10.1007/bf00910734

Source DB: PubMed Journal: Inflammation ISSN： 0360-3997 Impact factor: 4.092

11 in total

1. The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli.

Authors: E Schiffmann; H V Showell; B A Corcoran; P A Ward; E Smith; E L Becker
Journal: J Immunol Date: 1975-06 Impact factor: 5.422

2. Influence of corticosteroids on human polymorphonuclear leukocyte function in vitro : Reduction of lysosomal enzyme release and superoxide production.

Authors: I M Goldstein; D Roos; G Weissmann; H B Kaplan
Journal: Inflammation Date: 1976-06 Impact factor: 4.092

3. Effect of anti-inflammatory drugs and agents that elevate intracellular cyclic AMP on the release of toxic oxygen metabolites by phagocytes: studies in a model of tissue-bound IgG.

Authors: J E Lehmeyer; R B Johnston
Journal: Clin Immunol Immunopathol Date: 1978-04

4. Intracellular control of human neutrophil secretion. I. C5a-induced stimulus-specific desensitization and the effects of cytochalasin B.

Authors: P M Henson; B Zanolari; N A Schwartzman; S R Hong
Journal: J Immunol Date: 1978-09 Impact factor: 5.422

5. Chemotactic response to human C3a and C5a anaphylatoxins. I. Evaluation of C3a and C5a leukotaxis in vitro and under stimulated in vivo conditions.

Authors: H N Fernandez; P M Henson; A Otani; T E Hugli
Journal: J Immunol Date: 1978-01 Impact factor: 5.422

6. Mechanisms of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement.

Authors: I Goldstein; S Hoffstein; J Gallin; G Weissmann
Journal: Proc Natl Acad Sci U S A Date: 1973-10 Impact factor: 11.205

7. In vitro studies of immunologically induced secretion of mediators from cells and related phenomena.

Authors: E L Becker; P M Henson
Journal: Adv Immunol Date: 1973 Impact factor: 3.543

8. Partial characterization of human C5a anaphylatoxin. I. Chemical description of the carbohydrate and polypeptide prtions of human C5a.

Authors: H N Fernandez; T E Hugli
Journal: J Immunol Date: 1976-11 Impact factor: 5.422

9. Stimulation of human neutrophils by soluble and insoluble immunoglobulin aggregates. Secretion of granule constituents and increased oxidation of glucose.

Authors: P M Henson; Z G Oades
Journal: J Clin Invest Date: 1975-10 Impact factor: 14.808

10. The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal secretion for neutrophils.

Authors: H J Showell; R J Freer; S H Zigmond; E Schiffmann; S Aswanikumar; B Corcoran; E L Becker
Journal: J Exp Med Date: 1976-05-01 Impact factor: 14.307

14 in total

1. Differential effects of the complement peptides, C5a and C5a des Arg on human basophil and lung mast cell histamine release.

Authors: E S Schulman; T J Post; P M Henson; P C Giclas
Journal: J Clin Invest Date: 1988-03 Impact factor: 14.808

2. The erythrocyte as instigator of inflammation. Generation of amidated C3 by erythrocyte adenosine deaminase.

Authors: M K Hostetter; G M Johnson
Journal: J Clin Invest Date: 1989-08 Impact factor: 14.808

3. Eosinophil degranulation. Monitoring by interference contrast microscopy.

Authors: R Sher; A A Wadee
Journal: Inflammation Date: 1981-03 Impact factor: 4.092

4. Neutrophil-endothelial cell interactions. Modulation of neutrophil adhesiveness induced by complement fragments C5a and C5a des arg and formyl-methionyl-leucyl-phenylalanine in vitro.

Authors: M G Tonnesen; L A Smedly; P M Henson
Journal: J Clin Invest Date: 1984-11 Impact factor: 14.808

5. Analysis of the bimodal chemiluminescence pattern stimulated in human neutrophils by chemotactic factors.

Authors: J G Bender; D E Van Epps
Journal: Infect Immun Date: 1983-09 Impact factor: 3.441

6. Comparative study on biological activities of various anaphylatoxins (C4a, C3a, C5a). Investigations on their ability to induce platelet secretion.

Authors: S Meuer; T E Hugli; R H Andreatta; U Hadding; D Bitter-Suermann
Journal: Inflammation Date: 1981-12 Impact factor: 4.092

7. Altered function of synovial fluid granulocytes in patients with acute inflammatory arthritis: evidence for activation of neutrophils and its mediation by a factor present in synovial fluid.

Authors: J G Bender; D E Van Epps; R Searles; R C Williams
Journal: Inflammation Date: 1986-12 Impact factor: 4.092