Literature DB >> 21660687

Characterization of meiotic recombination initiation sites using pulsed-field gel electrophoresis.

Sarah Farmer1, Wing-Kit Leung, Hideo Tsubouchi.   

Abstract

High levels of homologous recombination are induced during meiosis. This meiotic recombination is initiated by programmed formation of DNA double-strand breaks (DSBs) by a conserved meiosis-specific protein, Spo11. Meiotic DSBs are not formed at random along chromosomes but are formed in clusters known as recombination hot spots. To understand the regulation of this initiation step of meiotic recombination, determining the timing and location of meiotic DSBs is essential. In this chapter, we describe a method to detect genome-wide meiotic DSBs by using a combination of pulsed-field gel electrophoresis and Southern blotting.

Entities:  

Mesh：

Year:  2011        PMID： 21660687     DOI： 10.1007/978-1-61779-129-1_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis.

Authors:  Bilge Argunhan; Wing-Kit Leung; Negar Afshar; Yaroslav Terentyev; Vijayalakshmi V Subramanian; Yasuto Murayama; Andreas Hochwagen; Hiroshi Iwasaki; Tomomi Tsubouchi; Hideo Tsubouchi
Journal:  EMBO J       Date:  2017-07-10       Impact factor: 11.598

2.  The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae.

Authors:  Santosh K Gothwal; Neem J Patel; Meaghan M Colletti; Hiroyuki Sasanuma; Miki Shinohara; Andreas Hochwagen; Akira Shinohara
Journal:  Genetics       Date:  2015-12-01       Impact factor: 4.562

3.  Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination.

Authors:  Sarah Farmer; Eun-Jin Erica Hong; Wing-Kit Leung; Bilge Argunhan; Yaroslav Terentyev; Neil Humphryes; Hiroshi Toyoizumi; Hideo Tsubouchi
Journal:  PLoS One       Date:  2012-06-22       Impact factor: 3.240

4.  Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast.

Authors:  Mohammad Bani Ismail; Miki Shinohara; Akira Shinohara
Journal:  PLoS One       Date:  2014-05-05       Impact factor: 3.240

5.  SCFCdc4 ubiquitin ligase regulates synaptonemal complex formation during meiosis.

Authors:  Zhihui Zhu; Mohammad Bani Ismail; Miki Shinohara; Akira Shinohara
Journal:  Life Sci Alliance       Date:  2020-12-08

6.  Direct and indirect control of the initiation of meiotic recombination by DNA damage checkpoint mechanisms in budding yeast.

Authors:  Bilge Argunhan; Sarah Farmer; Wing-Kit Leung; Yaroslav Terentyev; Neil Humphryes; Tomomi Tsubouchi; Hiroshi Toyoizumi; Hideo Tsubouchi
Journal:  PLoS One       Date:  2013-06-10       Impact factor: 3.240

7.  The Ecm11-Gmc2 complex promotes synaptonemal complex formation through assembly of transverse filaments in budding yeast.

Authors:  Neil Humphryes; Wing-Kit Leung; Bilge Argunhan; Yaroslav Terentyev; Martina Dvorackova; Hideo Tsubouchi
Journal:  PLoS Genet       Date:  2013-01-10       Impact factor: 5.917
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.