| Literature DB >> 2166042 |
K Lund1, A H Andersen, K Christiansen, J Q Svejstrup, O Westergaard.
Abstract
The minimal DNA requirement for topoisomerase II-mediated DNA cleavage in vitro was determined by analyzing the interaction of the enzyme with sets of DNA substrates varying successively by single bases at the 5'- or 3'-end of either strand. A 16-base pair double-stranded region was established as the minimal duplex region required for topoisomerase II cleavage activity. The region was located symmetrically around the 4-base staggered cleavage site. Topoisomerase II-mediated cleavage within the 16-base pair core duplex, however, required single-stranded regions flanking the duplex to either the 5'- or 3'-sides, or an extension at both ends of the duplex with 1 or more base pairs.Mesh：
Substances：
Year: 1990 PMID： 2166042
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157