Literature DB >> 21653778

Precision across the analytical measuring range of a quantitative real-time PCR assay for cytomegalovirus detection among three clinical laboratories.

Thomas E Grys1, Doreen L Duquette, Bruce White, Cole Irish, D Jane Hata, Bobbi S Pritt.   

Abstract

This study measured the precision of a quantitative laboratory-developed real-time PCR test for cytomegalovirus performed at three different clinical laboratories that use the same methodology. The overall standard deviation (adjusted for analyte level) was 0.18 log(10) copies/ml, and there was no significant relationship between standard deviation and analytical measuring range.

Mesh：

Year:  2011        PMID： 21653778      PMCID： PMC3147729          DOI： 10.1128/JCM.00486-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Comparative quantitation of cytomegalovirus (CMV) DNA in solid organ transplant recipients with CMV infection by using two high-throughput automated systems.

Authors:  R R Razonable; R A Brown; M J Espy; A Rivero; W Kremers; J Wilson; C Groettum; T F Smith; C V Paya
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

2.  Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0.

Authors:  J A Kellogg; P V Atria; J C Sanders; M E Eyster
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

3.  Multi-Site PCR-based CMV viral load assessment-assays demonstrate linearity and precision, but lack numeric standardization: a report of the association for molecular pathology.

Authors:  Daynna J Wolff; Denise Lamarche Heaney; Paul D Neuwald; Kathleen A Stellrecht; Richard D Press
Journal:  J Mol Diagn       Date:  2009-03       Impact factor: 5.568

4.  Interlaboratory comparison of epstein-barr virus viral load assays.

Authors:  J K Preiksaitis; X L Pang; J D Fox; J M Fenton; A M Caliendo; G G Miller
Journal:  Am J Transplant       Date:  2009-02       Impact factor: 8.086

5.  Interlaboratory comparison of cytomegalovirus viral load assays.

Authors:  X L Pang; J D Fox; J M Fenton; G G Miller; A M Caliendo; J K Preiksaitis
Journal:  Am J Transplant       Date:  2009-02       Impact factor: 8.086

6.  Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus.

Authors:  R T Hayden; K M Hokanson; S B Pounds; M J Bankowski; S W Belzer; J Carr; D Diorio; M S Forman; Y Joshi; D Hillyard; R L Hodinka; M N Nikiforova; C A Romain; J Stevenson; A Valsamakis; H H Balfour
Journal:  J Clin Microbiol       Date:  2007-11-07       Impact factor: 5.948

7.  A commutable cytomegalovirus calibrator is required to improve the agreement of viral load values between laboratories.

Authors:  Angela M Caliendo; Mona D Shahbazian; Carl Schaper; Jessica Ingersoll; Deborah Abdul-Ali; Jerry Boonyaratanakornkit; Xiao-Li Pang; Julie Fox; Jutta Preiksaitis; E Ralf Schönbrunner
Journal:  Clin Chem       Date:  2009-07-02       Impact factor: 8.327
  7 in total
  1 in total

1.  Monitoring of cytomegalovirus viral loads by two molecular assays in whole-blood and plasma samples from hematopoietic stem cell transplant recipients.

Authors:  N Esther Babady; Cindy Cheng; Evelyn Cumberbatch; Jeffrey Stiles; Genovefa Papanicolaou; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2015-02-04       Impact factor: 5.948
  1 in total

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