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Literature DB >> 2165109

Serotyping of human group A rotavirus with oligonucleotide probes.

O Sethabutr¹, L E Unicomb, I H Holmes, D N Taylor, R F Bishop, P Echeverria.

Abstract

Rotaviruses (RV) in stools of children with diarrhea in Thailand were serotyped by monoclonal enzyme immunoassay (MEIA), and RNA extracted from these specimens were tested for hybridization with oligonucleotides constructed from the nucleotide sequences of VP7 of human serotypes 1, 2, 3, and 4. Of 178 specimens that contained RV as identified with a monoclonal antibody to group A RV, 84% (149/178) hybridized with serotype-specific oligonucleotides, and 42% (74/178) were serotyped by MEIA (P less than .001). Of the 74 specimens that were serotyped by MEIA, 92% (35/38) of type 1, 97% (34/35) of type 2, and the one type 4 RV hybridized with the HuG1Ac, HuG2Ac, and HuG4Ac oligonucleotides, respectively. RV strains identified in children in Thailand in 1987 and 1988 to which a serotype could be assigned by either method were either type 1, type 2, or, less often, type 4. Testing RV for hybridization with oligonucleotides for genes encoding VP7 is an alternate method of determining RV serotypes.

Entities: Chemical Disease Species

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Year: 1990 PMID： 2165109 DOI： 10.1093/infdis/162.2.368

Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226

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Cited

13 in total

1. Evidence of high-frequency genomic reassortment of group A rotavirus strains in Bangladesh: emergence of type G9 in 1995.

Authors: L E Unicomb; G Podder; J R Gentsch; P A Woods; K Z Hasan; A S Faruque; M J Albert; R I Glass
Journal: J Clin Microbiol Date: 1999-06 Impact factor: 5.948

10. Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specificities, and serotype 2 VP7 genes.

Authors: F Bingnan; L E Unicomb; G L Tu; A Ali; A Malek; Z Rahim; S Tzipori
Journal: J Clin Microbiol Date: 1991-10 Impact factor: 5.948

Serotyping of human group A rotavirus with oligonucleotide probes.

1. Evidence of high-frequency genomic reassortment of group A rotavirus strains in Bangladesh: emergence of type G9 in 1995.

2. Identification of human and bovine rotavirus serotypes by polymerase chain reaction.

3. Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe.

4. Distribution of serotypes of human rotavirus in different populations.

5. Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR.

6. A one-year survey of rotavirus strains from three locations in Bangladesh.

7. Rotavirus diarrhea in Bangladeshi children: correlation of disease severity with serotypes.

8. Rotavirus infection detected in neonates from hospitals in urban Bangladesh.

9. Rotavirus-associated diarrhea in rural Bangladesh: two-year study of incidence and serotype distribution.

10. Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specificities, and serotype 2 VP7 genes.