| Literature DB >> 21650465 |
Ayumi Sumino1, Takehisa Dewa, Toshikazu Takeuchi, Ryuta Sugiura, Nobuaki Sasaki, Nobuo Misawa, Ryugo Tero, Tsuneo Urisu, Alastair T Gardiner, Richard J Cogdell, Hideki Hashimoto, Mamoru Nango.
Abstract
The construction and structural analysis of a tethered planar lipid bilayer containing bacterial photosynthetic membrane proteins, light-harvesting complex 2 (LH2), and light-harvesting core complex (LH1-RC) is described and establishes this system as an experimental platform for their functional analysis. The planar lipid bilayer containing LH2 and/or LH1-RC complexes was successfully formed on an avidin-immobilized coverglass via an avidin-biotin linkage. Atomic force microscopy (AFM) showed that a smooth continuous membrane was formed there. Lateral diffusion of these membrane proteins, observed by a fluorescence recovery after photobleaching (FRAP), is discussed in terms of the membrane architecture. Energy transfer from LH2 to LH1-RC within the tethered membrane was observed by steady-state fluorescence spectroscopy, indicating that the tethered membrane can mimic the natural situation.Entities:
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Year: 2011 PMID： 21650465 DOI： 10.1021/bm200585y
Source DB: PubMed Journal: Biomacromolecules ISSN： 1525-7797 Impact factor: 6.988