Literature DB >> 2164956

Expression of Pseudomonas phosphotriesterase activity in the fall armyworm confers resistance to insecticides.

D P Dumas1, J R Wild, F M Raushel.   

Abstract

The gene encoding for the phosphotriesterase (opd) from Pseudomonas diminuta has been subcloned into a baculovirus expression system. Functional enzyme is produced when the recombinant baculovirus is used to infect either cultured Spodoptera frugiperda sf9 cells or the larval stage of the fall armyworm. The LD50 for paraoxon toxicity was found to increase 280-fold in the larvae after infection with the recombinant baculovirus and expression of the functional phosphotriesterase.

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Year:  1990        PMID： 2164956     DOI： 10.1007/BF01939948

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  8 in total

1.  Graphical estimation of relative potency from quantal responses.

Authors:  D J FINNEY
Journal:  J Pharmacol Exp Ther       Date:  1952-04       Impact factor: 4.030

2.  An analysis of quantal response data in which the measurement of response is subject to error.

Authors:  J R Ashford; C S Smith
Journal:  Biometrics       Date:  1965-12       Impact factor: 2.571

Review 3.  Plasmid-determined resistance to antimicrobial drugs and toxic metal ions in bacteria.

Authors:  T J Foster
Journal:  Microbiol Rev       Date:  1983-09

4.  Structure-activity relationships in the hydrolysis of substrates by the phosphotriesterase from Pseudomonas diminuta.

Authors:  W J Donarski; D P Dumas; D P Heitmeyer; V E Lewis; F M Raushel
Journal:  Biochemistry       Date:  1989-05-30       Impact factor: 3.162

5.  Purification and properties of the phosphotriesterase from Pseudomonas diminuta.

Authors:  D P Dumas; S R Caldwell; J R Wild; F M Raushel
Journal:  J Biol Chem       Date:  1989-11-25       Impact factor: 5.157

6.  High level expression of nonfused foreign genes with Autographa californica nuclear polyhedrosis virus expression vectors.

Authors:  V A Luckow; M D Summers
Journal:  Virology       Date:  1989-05       Impact factor: 3.616

7.  Cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesterase.

Authors:  C S McDaniel; L L Harper; J R Wild
Journal:  J Bacteriol       Date:  1988-05       Impact factor: 3.490

8.  Mechanism and stereochemical course at phosphorus of the reaction catalyzed by a bacterial phosphotriesterase.

Authors:  V E Lewis; W J Donarski; J R Wild; F M Raushel
Journal:  Biochemistry       Date:  1988-03-08       Impact factor: 3.162
  8 in total
  2 in total

1.  Chemical biology: Catalytic detoxification.

Authors:  Frank M Raushel
Journal:  Nature       Date:  2011-01-20       Impact factor: 49.962

2.  Conformational variability of organophosphorus hydrolase upon soman and paraoxon binding.

Authors:  Diego E B Gomes; Roberto D Lins; Pedro G Pascutti; Chenghong Lei; Thereza A Soares
Journal:  J Phys Chem B       Date:  2011-12-05       Impact factor: 2.991
  2 in total

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