Literature DB >> 2164710

Inhibition of GTPase activating protein stimulation of Ras-p21 GTPase by the Krev-1 gene product.

M Frech1, J John, V Pizon, P Chardin, A Tavitian, R Clark, F McCormick, A Wittinghofer.   

Abstract

Krev-1 is known to suppress transformation by ras. However, the mechanism of the suppression is unclear. The protein product of Krev-1, Rap1A-p21, is identical to Ras-p21 proteins in the region where interaction with guanosine triphosphatase (GTPase) activating protein (GAP) is believed to occur. Therefore, the ability of GAP to interact with Rap1A-p21 was tested. Rap1A-p21 was not activated by GAP but bound tightly to GAP and was an effective competitive inhibitor of GAP-mediated Ras-GTPase activity. Binding of GAP to Rap1A-p21 was strictly guanosine triphosphate (GTP)-dependent. The ability of Rap1A-p21 to bind tightly to GAP may account for Krev-1 suppression of transformation by ras. This may occur by preventing interaction of GAP with Ras-p21 or with other cellular proteins necessary for GAP-mediated Ras GTPase activity.

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Year:  1990        PMID： 2164710     DOI： 10.1126/science.2164710

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  62 in total

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3.  Mutational and kinetic analyses of the GTPase-activating protein (GAP)-p21 interaction: the C-terminal domain of GAP is not sufficient for full activity.

Authors:  P Gideon; J John; M Frech; A Lautwein; R Clark; J E Scheffler; A Wittinghofer
Journal:  Mol Cell Biol       Date:  1992-05       Impact factor: 4.272

4.  Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS.

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Authors:  Y T Chen; C Holcomb; H P Moore
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-15       Impact factor: 11.205

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Review 9.  Recent advances in the understanding of interleukin-2 signal transduction.

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10.  Introduction of p130cas signaling complex formation upon integrin-mediated cell adhesion: a role for Src family kinases.

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Journal:  Mol Cell Biol       Date:  1996-06       Impact factor: 4.272
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