OBJECTIVE: To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium. DESIGN: Prospective, randomized study. SETTING: University research laboratory-based assisted reproductive technology laboratory. PATIENT(S): Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0-1, 2-3, 4-5, and 8-9 hours). Oocytes spindles in each group were immunostained for α-tubulin and chromosomes, and observed by confocal microscopy. MAIN OUTCOME MEASURE(S): Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly. RESULT(S): There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups. CONCLUSION(S): Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly.
RCT Entities:
OBJECTIVE: To develop and test a simple, feasible approach to improve spindle assembly and developmental competence of human in vitro matured oocytes by parthenogenetic activation in maturation medium. DESIGN: Prospective, randomized study. SETTING: University research laboratory-based assisted reproductive technology laboratory. PATIENT(S): Four hundred thirty-two patients with male factor infertility undergoing intracytoplasmic sperm injection (ICSI) cycles. INTERVENTION(S): In vivo failed-to-mature oocytes from the ICSI cycles were divided into four groups according to differing duration after the extrusion of first polar body (0-1, 2-3, 4-5, and 8-9 hours). Oocytes spindles in each group were immunostained for α-tubulin and chromosomes, and observed by confocal microscopy. MAIN OUTCOME MEASURE(S): Rate from pronuclear stage to blastocyst, embryo grading at the eight-cell and blastocyst stages, and spindle assembly. RESULT(S): There was a statistically significantly higher rate of development at the eight-cell and blastocyst stages in the 2- to 3-hour and 4- to 5-hour groups. The grading results at the eight-cell and blastocyst stages also showed that the proportion of embryos of high quality was similar among the 2- to 3-hour and 4- to 5-hour groups, and in these groups it was statistically significantly higher than the 0- to 1-hour and 8- to 9-hour groups. The results of immunofluorescence demonstrated that there was a statistically significantly higher rate of normal spindle assembly in the 2- to 3-hour and 4- to 5-hour groups. CONCLUSION(S): Optimal timing of maturation benefits the development of competence of in vitro matured oocytes by promoting normal spindle assembly.
Authors: Rachel S Mandelbaum; Michael S Awadalla; Meghan B Smith; Caroline J Violette; Brittany L Klooster; Rachel B Danis; Lynda K McGinnis; Jacqueline R Ho; Kristin A Bendikson; Richard J Paulson; Ali Ahmady Journal: J Assist Reprod Genet Date: 2021-06-24 Impact factor: 3.357