| Literature DB >> 21643787 |
Koji Yokoyama1, Masaharu Takahashi, Tsutomu Nishizawa, Shigeo Nagashima, Suljid Jirintai, Shigeru Yotsumoto, Hiroaki Okamoto, Mariko Y Momoi.
Abstract
A hepatitis C virus (HCV) strain (HC10-0804) recovered from a 12-year-old Japanese female with chronic hepatitis C segregated into discordant genotypes, 2b and 1b, in the 5'UTR/core and NS5B regions, respectively, thus suggesting an inter-genotypic recombination. The HC10-0804 isolate had a genomic length of 9,423 nucleotides (nt), excluding the poly(U) tract at the 3' terminus, and encoded a single open reading frame (ORF) for a polyprotein of 3,014 amino acids (aa). Based on Simplot and Bootscan analyses, the crossover point from 2b to 1b was estimated at nt 3443/3444 (aa 1034/1035), just after the beginning of the NS3 region. Comparison of the entire genomic sequence showed that the HC10-0804 strain was only 90.2% identical to the previously reported 2b/1b recombinant strain (SE-03-07-1689) from the Philippines, whose putative crossover point was 24 nt downstream of that of HC10-0804. These results indicate the circulation of a novel inter-genotypic (2b/1b) recombinant HCV in Japan.Entities:
Mesh：
Year: 2011 PMID： 21643787 DOI： 10.1007/s00705-011-1038-4
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574