Literature DB >> 21640767

Comparison of piggyBac transposition efficiency between linear and circular donor vectors in mammalian cells.

Hideyuki Nakanishi1, Yuriko Higuchi, Shigeru Kawakami, Fumiyoshi Yamashita, Mitsuru Hashida.   

Abstract

The piggyBac transposon has recently attracted attention as a tool for transgene integration in mammalian cells. However, previous studies involving piggyBac investigated only transposition from circular DNA, although some linear DNA vectors are used to transfect mammalian cells. In this study, we compared the transposition efficiency of piggyBac between linear and circular DNA. Colony counting assay, luciferase assay, and plasmid rescue assay showed that piggyBac transposon can transpose from linear DNA, but its efficiency is lower than the transposition efficiency from circular DNA. These results suggest that circular DNA is more suitable as donor vectors of piggyBac than linear DNA.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID： 21640767     DOI： 10.1016/j.jbiotec.2011.05.009

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  5 in total

Review 1.  Progress of Transposon Vector System for Production of Recombinant Therapeutic Proteins in Mammalian Cells.

Authors:  Mian Wei; Chun-Liu Mi; Chang-Qin Jing; Tian-Yun Wang
Journal:  Front Bioeng Biotechnol       Date:  2022-05-04

2.  The piggyBac-derived protein 5 (PGBD5) transposes both the closely and the distantly related piggyBac-like elements Tcr-pble and Ifp2.

Authors:  Laura Helou; Linda Beauclair; Hugues Dardente; Benoît Piégu; Louis Tsakou-Ngouafo; Thierry Lecomte; Alex Kentsis; Pierre Pontarotti; Yves Bigot
Journal:  J Mol Biol       Date:  2021-02-02       Impact factor: 5.469

3.  An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications.

Authors:  Wen Wen; Shanshan Song; Yuchun Han; Haibin Chen; Xiangzhen Liu; Qijun Qian
Journal:  Int J Mol Sci       Date:  2020-04-26       Impact factor: 5.923

4.  MAR elements and transposons for improved transgene integration and expression.

Authors:  Déborah Ley; Niamh Harraghy; Valérie Le Fourn; Solenne Bire; Pierre-Alain Girod; Alexandre Regamey; Florence Rouleux-Bonnin; Yves Bigot; Nicolas Mermod
Journal:  PLoS One       Date:  2013-04-30       Impact factor: 3.240

5.  The Functionality of Minimal PiggyBac Transposons in Mammalian Cells.

Authors:  Boris Troyanovsky; Vira Bitko; Viktor Pastukh; Brian Fouty; Victor Solodushko
Journal:  Mol Ther Nucleic Acids       Date:  2016-10-04       Impact factor: 10.183
  5 in total

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