Literature DB >> 21639392

Determination of lipophilicity by gradient elution high-performance liquid chromatography.

J D Krass1, B Jastorff, H G Genieser.   

Abstract

A novel method for the determination of lipophilicity using a simple HPLC protocol based on gradient elution chromatography is presented and compared to the common isocratic log k'(w) procedure. Linear relationships with high correlation coefficients between both methods for biologically active nucleosides and cyclic nucleotides as well as for environmentally relevant aromatic hydrocarbons were found. A mathematical fit to support the empirically determined linear relationship is presented. It is shown that the observed relationship between log k'(w) and the apparent capacity factor (k'(g)) determined by gradient elution is derivable by theoretical considerations as well. Since the gradient method is much less time-consuming compared to other procedures, it represents a convenient alternative for determining lipophilicity data in the future.

Entities:  

Year:  1997        PMID： 21639392     DOI： 10.1021/ac961246i

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  8 in total

1.  Quantification of cAMP and cGMP analogs in intact cells: pitfalls in enzyme immunoassays for cyclic nucleotides.

Authors:  Katharina Werner; Frank Schwede; Hans-Gottfried Genieser; Jörg Geiger; Elke Butt
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2011-06-29       Impact factor: 3.000

2.  Synthesis and in vitro evaluation of oxindole derivatives as potential radioligands for 5-HT(7) receptor imaging with PET.

Authors:  Matthias M Herth; Balázs Volk; Katalin Pallagi; Lasse Kofoed Bech; Ferenc A Antoni; Gitte M Knudsen; Jesper L Kristensen
Journal:  ACS Chem Neurosci       Date:  2012-08-31       Impact factor: 4.418

3.  Hydrolysis products of cAMP analogs cause transformation of Trypanosoma brucei from slender to stumpy-like forms.

Authors:  Sunil Laxman; Aaron Riechers; Martin Sadilek; Frank Schwede; Joseph A Beavo
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-01       Impact factor: 11.205

Review 4.  Regulating the ubiquitin/proteasome pathway via cAMP-signaling: neuroprotective potential.

Authors:  He Huang; Hu Wang; Maria E Figueiredo-Pereira
Journal:  Cell Biochem Biophys       Date:  2013-09       Impact factor: 2.194

5.  Manganese-Based Contrast Agents for Magnetic Resonance Imaging of Liver Tumors: Structure-Activity Relationships and Lead Candidate Evaluation.

Authors:  Junfeng Wang; Huan Wang; Ian A Ramsay; Derek J Erstad; Bryan C Fuchs; Kenneth K Tanabe; Peter Caravan; Eric M Gale
Journal:  J Med Chem       Date:  2018-09-25       Impact factor: 7.446

6.  Biochemical characterization and cellular imaging of a novel, membrane permeable fluorescent cAMP analog.

Authors:  Daniela Moll; Anke Prinz; Cornelia M Brendel; Marco Berrera; Katrin Guske; Manuela Zaccolo; Hans-Gottfried Genieser; Friedrich W Herberg
Journal:  BMC Biochem       Date:  2008-06-25       Impact factor: 4.059

7.  PKA-RII subunit phosphorylation precedes activation by cAMP and regulates activity termination.

Authors:  Jörg Isensee; Melanie Kaufholz; Matthias J Knape; Jan Hasenauer; Hanna Hammerich; Humberto Gonczarowska-Jorge; René P Zahedi; Frank Schwede; Friedrich W Herberg; Tim Hucho
Journal:  J Cell Biol       Date:  2018-04-03       Impact factor: 10.539

8.  In Vitro and In Vivo Inhibition of the Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT by Amidinoureas.

Authors:  Samantha Ottavi; Sarah M Scarry; John Mosior; Yan Ling; Julia Roberts; Amrita Singh; David Zhang; Laurent Goullieux; Christine Roubert; Eric Bacqué; H Rachel Lagiakos; Jeremie Vendome; Francesca Moraca; Kelin Li; Andrew J Perkowski; Remya Ramesh; Matthew M Bowler; William Tracy; Victoria A Feher; James C Sacchettini; Ben S Gold; Carl F Nathan; Jeffrey Aubé
Journal:  J Med Chem       Date:  2022-01-19       Impact factor: 8.039
  8 in total

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