Literature DB >> 21636991

Escherichia coli K5 heparosan fermentation and improvement by genetic engineering.

Zhenyu Wang1, Jonathan S Dordick, Robert J Linhardt.   

Abstract

N-acetyl heparosan is the precursor for the biosynthesis of the important anticoagulant drug heparin. The E. coli K5 capsular heparosan polysaccharide provides a promising precursor for in vitro chemoenzymatic production of bioengineered heparin. This article explores the improvements of heparosan production for bioengineered heparin by fermentation process engineering and genetic engineering.
© 2011 Landes Bioscience

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Year:  2011        PMID： 21636991      PMCID： PMC3127022          DOI： 10.4161/bbug.2.1.14201

Source DB:  PubMed          Journal:  Bioeng Bugs        ISSN： 1949-1018


  34 in total

1.  Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events.

Authors:  Marco Guerrini; Daniela Beccati; Zachary Shriver; Annamaria Naggi; Karthik Viswanathan; Antonella Bisio; Ishan Capila; Jonathan C Lansing; Sara Guglieri; Blair Fraser; Ali Al-Hakim; Nur Sibel Gunay; Zhenqing Zhang; Luke Robinson; Lucinda Buhse; Moheb Nasr; Janet Woodcock; Robert Langer; Ganesh Venkataraman; Robert J Linhardt; Benito Casu; Giangiacomo Torri; Ram Sasisekharan
Journal:  Nat Biotechnol       Date:  2008-04-23       Impact factor: 54.908

2.  Identification that KfiA, a protein essential for the biosynthesis of the Escherichia coli K5 capsular polysaccharide, is an alpha -UDP-GlcNAc glycosyltransferase. The formation of a membrane-associated K5 biosynthetic complex requires KfiA, KfiB, and KfiC.

Authors:  N Hodson; G Griffiths; N Cook; M Pourhossein; E Gottfridson; T Lind; K Lidholt; I S Roberts
Journal:  J Biol Chem       Date:  2000-09-01       Impact factor: 5.157

3.  An E. coli expression system for the extracellular secretion of barley alpha-amylase.

Authors:  C C Lee; D W Wong; G H Robertson
Journal:  J Protein Chem       Date:  2001-04

Review 4.  ABC transporters and the export of capsular polysaccharides from gram-negative bacteria.

Authors:  R P Silver; K Prior; C Nsahlai; L F Wright
Journal:  Res Microbiol       Date:  2001 Apr-May       Impact factor: 3.992

5.  Analysis of E. coli K5 capsular polysaccharide heparosan.

Authors:  Mellisa Ly; Zhenyu Wang; Tatiana N Laremore; Fuming Zhang; Weihong Zhong; Dennis Pu; Dmitri V Zagorevski; Jonathan S Dordick; Robert J Linhardt
Journal:  Anal Bioanal Chem       Date:  2010-04-21       Impact factor: 4.142

6.  The transport of group 2 capsular polysaccharides across the periplasmic space in Escherichia coli. Roles for the KpsE and KpsD proteins.

Authors:  C Arrecubieta; T C Hammarton; B Barrett; S Chareonsudjai; N Hodson; D Rainey; I S Roberts
Journal:  J Biol Chem       Date:  2000-11-14       Impact factor: 5.157

7.  E. coli K5 fermentation and the preparation of heparosan, a bioengineered heparin precursor.

Authors:  Zhenyu Wang; Mellisa Ly; Fuming Zhang; Weihong Zhong; Amy Suen; Anne Marie Hickey; Jonathan S Dordick; Robert J Linhardt
Journal:  Biotechnol Bioeng       Date:  2010-12-15       Impact factor: 4.530

8.  Contaminated heparin associated with adverse clinical events and activation of the contact system.

Authors:  Takashi Kei Kishimoto; Karthik Viswanathan; Tanmoy Ganguly; Subbiah Elankumaran; Sean Smith; Kevin Pelzer; Jonathan C Lansing; Nammalwar Sriranganathan; Ganlin Zhao; Zoya Galcheva-Gargova; Ali Al-Hakim; Gregory Scott Bailey; Blair Fraser; Sucharita Roy; Thomas Rogers-Cotrone; Lucinda Buhse; Mark Whary; James Fox; Moheb Nasr; Gerald J Dal Pan; Zachary Shriver; Robert S Langer; Ganesh Venkataraman; K Frank Austen; Janet Woodcock; Ram Sasisekharan
Journal:  N Engl J Med       Date:  2008-04-23       Impact factor: 91.245

9.  Glucuronyltransferase activity of KfiC from Escherichia coli strain K5 requires association of KfiA: KfiC and KfiA are essential enzymes for production of K5 polysaccharide, N-acetylheparosan.

Authors:  Nobuo Sugiura; Yuichi Baba; Yoshirou Kawaguchi; Toru Iwatani; Kiyoshi Suzuki; Takahiro Kusakabe; Kiwamu Yamagishi; Koji Kimata; Yoshimitsu Kakuta; Hideto Watanabe
Journal:  J Biol Chem       Date:  2009-11-13       Impact factor: 5.157

Review 10.  Lessons learned from the contamination of heparin.

Authors:  Haiying Liu; Zhenqing Zhang; Robert J Linhardt
Journal:  Nat Prod Rep       Date:  2009-01-19       Impact factor: 13.423
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  16 in total

1.  Cyclic AMP (cAMP) Receptor Protein-cAMP Complex Regulates Heparosan Production in Escherichia coli Strain Nissle 1917.

Authors:  Huihui Yan; Feifei Bao; Liping Zhao; Yanying Yu; Jiaqin Tang; Xianxuan Zhou
Journal:  Appl Environ Microbiol       Date:  2015-08-28       Impact factor: 4.792

2.  New Means to Control Molecular Assembly.

Authors:  Jiali Zhang; Hai Yu; Bradley Harris; Yunbo Zheng; Umit Celik; Lan Na; Roland Faller; Xi Chen; Dominik R Haudenschild; Gang-Yu Liu
Journal:  J Phys Chem C Nanomater Interfaces       Date:  2020-02-23       Impact factor: 4.126

3.  Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin.

Authors:  Zhenyu Wang; Jennifer Li; Samantha Cheong; Ujjwal Bhaskar; Onishi Akihiro; Fuming Zhang; Jonathan S Dordick; Robert J Linhardt
Journal:  J Biotechnol       Date:  2011-09-10       Impact factor: 3.307

Review 4.  Engineering of routes to heparin and related polysaccharides.

Authors:  Ujjwal Bhaskar; Eric Sterner; Anne Marie Hickey; Akihiro Onishi; Fuming Zhang; Jonathan S Dordick; Robert J Linhardt
Journal:  Appl Microbiol Biotechnol       Date:  2011-11-03       Impact factor: 4.813

5.  Nanoparticle Surface Engineering with Heparosan Polysaccharide Reduces Serum Protein Adsorption and Enhances Cellular Uptake.

Authors:  Wen Yang; Lin Wang; Mulin Fang; Vinit Sheth; Yushan Zhang; Alyssa M Holden; Nathan D Donahue; Dixy E Green; Alex N Frickenstein; Evan M Mettenbrink; Tyler A Schwemley; Emmy R Francek; Majood Haddad; Md Nazir Hossen; Shirsha Mukherjee; Si Wu; Paul L DeAngelis; Stefan Wilhelm
Journal:  Nano Lett       Date:  2022-02-15       Impact factor: 12.262

6.  Molecular Mass Characterization of Glycosaminoglycans with Different Degrees of Sulfation in Bioengineered Heparin Process by Size Exclusion Chromatography.

Authors:  Zhenyu Wang; Fuming Zhang; Jonathan S Dordick; Robert J Linhardt
Journal:  Curr Anal Chem       Date:  2012-10-01       Impact factor: 1.892

Review 7.  Chemoenzymatic synthesis of glycosaminoglycans: re-creating, re-modeling and re-designing nature's longest or most complex carbohydrate chains.

Authors:  Paul L DeAngelis; Jian Liu; Robert J Linhardt
Journal:  Glycobiology       Date:  2013-03-11       Impact factor: 4.313

8.  High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production.

Authors:  Odile Francesca Restaino; Ujjwal Bhaskar; Priscilla Paul; Lingyun Li; Mario De Rosa; Jonathan S Dordick; Robert J Linhardt
Journal:  Appl Microbiol Biotechnol       Date:  2013-01-15       Impact factor: 4.813

9.  Assays for determining heparan sulfate and heparin O-sulfotransferase activity and specificity.

Authors:  Eric Sterner; Lingyun Li; Priscilla Paul; Julie M Beaudet; Jian Liu; Robert J Linhardt; Jonathan S Dordick
Journal:  Anal Bioanal Chem       Date:  2013-11-23       Impact factor: 4.142

Review 10.  Bioengineered heparins and heparan sulfates.

Authors:  Li Fu; Matthew Suflita; Robert J Linhardt
Journal:  Adv Drug Deliv Rev       Date:  2015-11-10       Impact factor: 15.470
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