A simple, sensitive, rapid and specific detection method for Bisphenol A based on Fluorescence Polarization Immunoassay.

A rapid, sensitive, specific and simple method based on Fluorescence Polarization Immunoassay (FPIA) was developed for the quantitative detection of Bisphenol A in water. 4, 4-Bis (4-hydroxyphenyl) valeric acid (BHPVA) was selected as the hapten to produce the immunogen. Fluorescein-labeled Bisphenol A derivatives (tracers) with different structures were synthesized and purified by thin layer chromatography. Based on the polyclonal antibody and tracers, an optimized FPIA method was developed with a detection limit (10% inhibition) of 2 μg L(-1) and a linear working range of 20 to 800 μg L(-1). FPIA was suitable for screening a large number of samples. The recoveries of fortified Tai lake water samples ranged from 91.85 to 102.78%, and tap water samples ranged from 90.36 to 96.01%. The coefficients of variation were all less than 20%. This FPIA method, which did not require a complicated cleanup process, proved to be very useful for the screening of Bisphenol A in environmental water samples.